PMID- 11227190
OWN - NLM
STAT- MEDLINE
DCOM- 20010823
LR  - 20191210
IS  - 0830-9000 (Print)
IS  - 0830-9000 (Linking)
VI  - 65
IP  - 1
DP  - 2001 Jan
TI  - An evaluation of test and removal for the elimination of porcine reproductive and 
      respiratory syndrome virus from 5 swine farms.
PG  - 22-7
AB  - The objective of this field study was to evaluate the protocol of test and 
      removal (T&R) for the elimination of porcine reproductive and respiratory 
      syndrome virus (PRRSV) from 5 chronically infected breeding herds. The T&R 
      protocol involved sampling the entire breeding herd in one day, testing sera by 
      polymerase chain reaction and ELISA to detect previously exposed and/or infected 
      animals, and subsequently removing them from the herd. Following completion of 
      T&R, breeding herds were monitored for 12 consecutive months, using ELISA, for 
      the presence of antibodies to PRRSV. In order to be classified as a 
      PRRSV-negative herd, all samples collected over the 12-month monitoring period 
      were required to be negative by ELISA (s/p ratio < 0.4). At the conclusion of the 
      monitoring period, all 5 farms were PRRSV-negative, according to the defined 
      testing criteria. Approximately 2.2% (74/3408) ELISA false positive samples were 
      detected across all 5 farms during the monitoring period. The diagnostic cost 
      required during the T&R protocol was approximately US $10.66 per animal tested. 
      Limitations of the study were a lack of herds with large (> 2000 sows) breeding 
      herd inventories, and herds with a history of PRRSV vaccination.
FAU - Dee, S A
AU  - Dee SA
AD  - Department of Clinical and Population Sciences, University of Minnesota College 
      of Veterinary Medicine, St. Paul 55108, USA. deexx004@tc.umn.edu
FAU - Bierk, M D
AU  - Bierk MD
FAU - Deen, J
AU  - Deen J
FAU - Molitor, T W
AU  - Molitor TW
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Canada
TA  - Can J Vet Res
JT  - Canadian journal of veterinary research = Revue canadienne de recherche 
      veterinaire
JID - 8607793
SB  - IM
MH  - *Animal Husbandry/economics/methods
MH  - Animals
MH  - Costs and Cost Analysis
MH  - Enzyme-Linked Immunosorbent Assay/veterinary
MH  - False Positive Reactions
MH  - Pilot Projects
MH  - Polymerase Chain Reaction/veterinary
MH  - Porcine Reproductive and Respiratory Syndrome/*diagnosis/epidemiology/prevention 
      & control
MH  - Porcine respiratory and reproductive syndrome virus/immunology/*isolation & 
      purification
MH  - Sensitivity and Specificity
MH  - Swine
MH  - Time Factors
MH  - Vaccination/veterinary
PMC - PMC1189637
EDAT- 2001/03/03 10:00
MHDA- 2001/08/24 10:01
PMCR- 2001/04/01
CRDT- 2001/03/03 10:00
PHST- 2001/03/03 10:00 [pubmed]
PHST- 2001/08/24 10:01 [medline]
PHST- 2001/03/03 10:00 [entrez]
PHST- 2001/04/01 00:00 [pmc-release]
PST - ppublish
SO  - Can J Vet Res. 2001 Jan;65(1):22-7.