PMID- 11227544
OWN - NLM
STAT- MEDLINE
DCOM- 20010419
LR  - 20191025
IS  - 0937-0633 (Print)
IS  - 0937-0633 (Linking)
VI  - 368
IP  - 7
DP  - 2000 Dec
TI  - Measurements for certification of chlortetracycline reference materials within 
      the European Union Standards, Measurements and Testing programme.
PG  - 656-63
AB  - The experimental design of a material certification programme is described. The 
      matrix reference materials (RMs) comprised chlortetracycline (CTC)-containing and 
      CTC-free lyophilised porcine liver, kidney and muscle produced under the European 
      Commission's Standards Measurements and Testing (SMT) programme. The aim of the 
      certification programme was to determine accurately and precisely the 
      concentration of CTC and 4-epi-chlortetracycline (epi-CTC) contained in the RMs. 
      A multi-laboratory approach was used to certify analyte concentrations. 
      Participants (n = 19) were instructed to strictly adhere to previously 
      established guidelines. Following the examination of analytical performance 
      criteria, statistical manipulation of results submitted by 13 laboratories, (6 
      withdrew) allowed an estimate to be made of the true value of the analyte 
      content. The Nalimov test was used for detection of outlying results. The Cochran 
      and Bartlett tests were employed for testing the homogeneity of variances. The 
      normality of results distribution was tested according to the 
      Kolmogorov-Smirnov-Lilliefors test. One-way analysis of variance (ANOVA) was 
      employed to calculate the within and between-laboratory standard deviations, the 
      overall mean and confidence interval for the CTC and epi-CTC content of each of 
      the RMs. Certified values were within or very close to the target concentration 
      ranges specified in the SMT contract. These studies have demonstrated the 
      successful production and certification of CTC-containing and CTC-free porcine 
      RMs.
FAU - Juhel-Gaugain, M
AU  - Juhel-Gaugain M
AD  - Community Reference Laboratory, AFSSA-Fougeres, France. m.juhel@fougeres.afssa.fr
FAU - McEvoy, J D
AU  - McEvoy JD
FAU - VanGinkel, L A
AU  - VanGinkel LA
LA  - eng
PT  - Journal Article
PL  - Germany
TA  - Fresenius J Anal Chem
JT  - Fresenius' journal of analytical chemistry
JID - 9114077
RN  - 0 (Anti-Bacterial Agents)
RN  - WCK1KIQ23Q (Chlortetracycline)
SB  - IM
MH  - Animals
MH  - Anti-Bacterial Agents/*analysis/*standards
MH  - Chlortetracycline/*analysis/*standards
MH  - Data Interpretation, Statistical
MH  - Europe
MH  - Humans
MH  - Kidney/chemistry
MH  - Liver/chemistry
MH  - Muscle, Skeletal/chemistry
MH  - Reference Standards
EDAT- 2001/03/03 10:00
MHDA- 2001/04/21 10:01
CRDT- 2001/03/03 10:00
PHST- 2001/03/03 10:00 [pubmed]
PHST- 2001/04/21 10:01 [medline]
PHST- 2001/03/03 10:00 [entrez]
AID - 10.1007/s002160000570 [doi]
PST - ppublish
SO  - Fresenius J Anal Chem. 2000 Dec;368(7):656-63. doi: 10.1007/s002160000570.