PMID- 11228167
OWN - NLM
STAT- MEDLINE
DCOM- 20010705
LR  - 20220215
IS  - 0021-9533 (Print)
IS  - 0021-9533 (Linking)
VI  - 114
IP  - Pt 6
DP  - 2001 Mar
TI  - Mutant cadherin affects epithelial morphogenesis and invasion, but not 
      transformation.
PG  - 1237-46
AB  - MDCK cells were engineered to reversibly express mutant E-cadherin protein with a 
      large extracellular deletion. Mutant cadherin overexpression reduced the 
      expression of endogenous E- and K-cadherins in MDCK cells to negligible levels, 
      resulting in decreased cell adhesion. Despite severe impairment of the cadherin 
      adhesion system, cells overexpressing mutant E-cadherin formed fluid-filled cysts 
      in collagen gel cultures and responded to hepatocyte growth factor/scatter factor 
      (HGF/SF) that induced cellular extension formation with a frequency similar to 
      that of control cysts. However, cells were shed from cyst walls into the lumen 
      and into the collagen matrix prior to and during HGF/SF induced tubule extension. 
      Despite the propensity for cell dissociation, MDCK cells lacking cadherin 
      adhesion molecules were not capable of anchorage-independent growth in soft agar 
      and cell proliferation rate was not affected. Thus, cadherin loss does not induce 
      transformation, despite inducing an invasive phenotype, a later stage of tumor 
      progression. These experiments are especially relevant to tumor progression in 
      cells with altered E-cadherin expression, particularly tumor samples with 
      identified E-cadherin extracellular domain genomic mutations.
FAU - Troxell, M L
AU  - Troxell ML
AD  - Department of Molecular and Cellular Physiology, Beckman Center B109, Stanford 
      University School of Medicine, Stanford, CA 94305-5426, USA.
FAU - Loftus, D J
AU  - Loftus DJ
FAU - Nelson, W J
AU  - Nelson WJ
FAU - Marrs, J A
AU  - Marrs JA
LA  - eng
GR  - DK-54518/DK/NIDDK NIH HHS/United States
GR  - EY-11365/EY/NEI NIH HHS/United States
GR  - GM-07365/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - J Cell Sci
JT  - Journal of cell science
JID - 0052457
RN  - 0 (Cadherins)
RN  - 0 (Gels)
RN  - 67256-21-7 (Hepatocyte Growth Factor)
RN  - 9002-18-0 (Agar)
RN  - 9007-34-5 (Collagen)
SB  - IM
MH  - Agar
MH  - Animals
MH  - Cadherins/*biosynthesis/genetics
MH  - Cell Aggregation
MH  - Cell Division
MH  - Cell Line
MH  - Collagen
MH  - Dogs
MH  - Epithelial Cells/cytology/drug effects/*physiology
MH  - Gels
MH  - Gene Expression
MH  - Hepatocyte Growth Factor/metabolism/pharmacology
MH  - Humans
MH  - Morphogenesis
MH  - Mutagenesis
MH  - *Transformation, Genetic
EDAT- 2001/03/03 10:00
MHDA- 2001/07/06 10:01
CRDT- 2001/03/03 10:00
PHST- 2001/03/03 10:00 [pubmed]
PHST- 2001/07/06 10:01 [medline]
PHST- 2001/03/03 10:00 [entrez]
AID - 10.1242/jcs.114.6.1237 [doi]
PST - ppublish
SO  - J Cell Sci. 2001 Mar;114(Pt 6):1237-46. doi: 10.1242/jcs.114.6.1237.