PMID- 11228246
OWN - NLM
STAT- MEDLINE
DCOM- 20010628
LR  - 20190513
IS  - 1360-9947 (Print)
IS  - 1360-9947 (Linking)
VI  - 7
IP  - 3
DP  - 2001 Mar
TI  - Effects of interleukin-4 on the in-vitro production of cytokines by human 
      endometrial stromal cells.
PG  - 265-70
AB  - A T helper (Th)1/Th2 model has been applied to as a system regulating the 
      cytokine network during pregnancy. To evaluate the effects of interleukin (IL)-4, 
      a Th2 cytokine, on the cytokine production by endometrial stromal cells (ESC), an 
      enzyme-linked immunosorbent assay was used to measure the concentrations of IL-6, 
      IL-8, monocyte chemoattractant protein-1 (MCP-1), and macrophage 
      colony-stimulating factor (M-CSF) in the culture media of ESC and of an 
      endometrial stromal sarcoma cell line, MaMi, following the addition of 
      recombinant human IL-4. The expression of mRNAs for IL-6 and IL-8 in ESC after 
      stimulation with IL-4 was also evaluated by Northern blot analysis. Increases in 
      the concentrations of IL-6, IL-8, MCP-1, and M-CSF in the culture media of ESC 
      and MaMi cells were observed on the addition of increasing amounts of IL-4. This 
      cytokine also stimulated the transcription of IL-6 and IL-8 in ESC in a 
      dose-dependent manner. It is suggested that IL-4 secreted by the maternal 
      decidual tissue as well as by the developing embryo may stimulate the production 
      of IL-6, IL-8, MCP-1, and M-CSF by ESC. The increased concentration of these 
      cytokines in the local environment may contribute to the maintenance of early 
      pregnancy by modulating the immune reaction at the feto-maternal interface.
FAU - Nasu, K
AU  - Nasu K
AD  - Department of Obstetrics and Gynecology, Oita Medical University, Hasama-machi, 
      Oita 879-5593, Japan. NASU@oita-med.ac.jp
FAU - Sugano, T
AU  - Sugano T
FAU - Fujisawa, K
AU  - Fujisawa K
FAU - Arima, K
AU  - Arima K
FAU - Narahara, H
AU  - Narahara H
FAU - Miyakawa, I
AU  - Miyakawa I
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Mol Hum Reprod
JT  - Molecular human reproduction
JID - 9513710
RN  - 0 (Chemokine CCL2)
RN  - 0 (Culture Media)
RN  - 0 (Interleukin-6)
RN  - 0 (Interleukin-8)
RN  - 0 (RNA, Messenger)
RN  - 207137-56-2 (Interleukin-4)
RN  - 81627-83-0 (Macrophage Colony-Stimulating Factor)
SB  - IM
MH  - Animals
MH  - Chemokine CCL2/*biosynthesis
MH  - Culture Media
MH  - Endometrium/cytology
MH  - Female
MH  - Gene Expression
MH  - Humans
MH  - Interleukin-4/*immunology/pharmacology
MH  - Interleukin-6/*biosynthesis/genetics
MH  - Interleukin-8/*biosynthesis/genetics
MH  - Macrophage Colony-Stimulating Factor/*biosynthesis
MH  - RNA, Messenger
MH  - Stromal Cells/drug effects/*immunology
EDAT- 2001/03/03 10:00
MHDA- 2001/06/29 10:01
CRDT- 2001/03/03 10:00
PHST- 2001/03/03 10:00 [pubmed]
PHST- 2001/06/29 10:01 [medline]
PHST- 2001/03/03 10:00 [entrez]
AID - 10.1093/molehr/7.3.265 [doi]
PST - ppublish
SO  - Mol Hum Reprod. 2001 Mar;7(3):265-70. doi: 10.1093/molehr/7.3.265.