PMID- 11229465
OWN - NLM
STAT- MEDLINE
DCOM- 20010322
LR  - 20181130
IS  - 0004-3591 (Print)
IS  - 0004-3591 (Linking)
VI  - 44
IP  - 2
DP  - 2001 Feb
TI  - Effects of intraarticular glucocorticoids on macrophage infiltration and 
      mediators of joint damage in osteoarthritis synovial membranes: findings in a 
      double-blind, placebo-controlled study.
PG  - 343-50
AB  - OBJECTIVE: To investigate the effects of intraarticular glucocorticoid treatment 
      on macrophage infiltration, the expression of the chemokines monocyte 
      chemoattractant protein 1 (MCP-1) and macrophage inflammatory protein 1alpha 
      (MIP-1alpha), and the expression of matrix metalloproteinases 1 and 3 (MMPs 1 and 
      3) and their inhibitors, the tissue inhibitors of metalloproteinases 1 and 2 
      (TIMPs 1 and 2), in osteoarthritis (OA) synovial membranes. METHODS: Forty 
      patients underwent arthroscopic biopsy before and 1 month after intraarticular 
      injection of glucocorticoids. Twenty-one patients received 120 mg of 
      methylprednisolone acetate (Depo-Medrol; Upjohn, Kalamazoo, MI), and 20 patients 
      received placebo (1 patient received placebo in 1 knee and methylprednisolone 
      acetate in the other). Immunoperoxidase staining for the expression of CD68, 
      MCP-1, MIP-1alpha, MMP-1, MMP-3, TIMP-1, and TIMP-2 was performed, and the 
      immunostaining was quantified by color video image analysis. RESULTS: CD68, 
      MCP-1, MIP-1alpha, MMP-1, MMP-3, TIMP-1, and TIMP-2 immunostaining was observed 
      in all synovial membranes. Intraarticular glucocorticoid treatment was associated 
      with a small (30%) but statistically significant (P = 0.048) reduction in CD68+ 
      macrophage staining in the synovial lining layer, but there was no change in the 
      CD68 expression in the synovial sublining layer. No significant differences were 
      observed for MCP-1, MIP-1alpha, MMP-1, MMP-3, TIMP-1, and TIMP-2 immunostaining 
      in the synovial lining or sublining layers. CONCLUSION: Intraarticular 
      glucocorticoids may reduce CD68+ macrophage infiltration into the synovial lining 
      layer, but not the expression of MCP-1, MIP-1alpha, MMP-1, MMP-3, TIMP-1, and 
      TIMP-2 in the synovial membrane, in patients with OA.
FAU - Young, L
AU  - Young L
AD  - Prince of Wales Hospital, Sydney, New South Wales, Australia.
FAU - Katrib, A
AU  - Katrib A
FAU - Cuello, C
AU  - Cuello C
FAU - Vollmer-Conna, U
AU  - Vollmer-Conna U
FAU - Bertouch, J V
AU  - Bertouch JV
FAU - Roberts-Thomson, P J
AU  - Roberts-Thomson PJ
FAU - Ahern, M J
AU  - Ahern MJ
FAU - Smith, M D
AU  - Smith MD
FAU - Youssef, P P
AU  - Youssef PP
LA  - eng
PT  - Clinical Trial
PT  - Journal Article
PT  - Randomized Controlled Trial
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Arthritis Rheum
JT  - Arthritis and rheumatism
JID - 0370605
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemokine CCL3)
RN  - 0 (Chemokine CCL4)
RN  - 0 (Glucocorticoids)
RN  - 0 (Inflammation Mediators)
RN  - 0 (Macrophage Inflammatory Proteins)
RN  - 0 (Matrix Metalloproteinase Inhibitors)
RN  - 0 (Placebos)
RN  - 0 (Tissue Inhibitor of Metalloproteinase-1)
RN  - 127497-59-0 (Tissue Inhibitor of Metalloproteinase-2)
RN  - 43502P7F0P (Methylprednisolone Acetate)
RN  - EC 3.4.24.- (Matrix Metalloproteinases, Membrane-Associated)
RN  - EC 3.4.24.- (Metalloendopeptidases)
RN  - EC 3.4.24.7 (Matrix Metalloproteinase 1)
RN  - X4W7ZR7023 (Methylprednisolone)
SB  - IM
MH  - Aged
MH  - Cell Movement/drug effects
MH  - Chemokine CCL2/biosynthesis
MH  - Chemokine CCL3
MH  - Chemokine CCL4
MH  - Double-Blind Method
MH  - Female
MH  - Glucocorticoids/*administration & dosage/pharmacology
MH  - Humans
MH  - Immunohistochemistry
MH  - Inflammation Mediators/pharmacology
MH  - Injections, Intra-Articular
MH  - Macrophage Inflammatory Proteins/biosynthesis
MH  - Macrophages/cytology
MH  - Male
MH  - Matrix Metalloproteinase 1/biosynthesis
MH  - Matrix Metalloproteinase Inhibitors
MH  - Matrix Metalloproteinases, Membrane-Associated
MH  - Metalloendopeptidases/antagonists & inhibitors/biosynthesis
MH  - Methylprednisolone/*analogs & derivatives
MH  - Methylprednisolone Acetate
MH  - Middle Aged
MH  - Osteoarthritis/pathology
MH  - Placebos
MH  - Synovial Membrane/chemistry/drug effects
MH  - Time Factors
MH  - Tissue Inhibitor of Metalloproteinase-1/biosynthesis
MH  - Tissue Inhibitor of Metalloproteinase-2/biosynthesis
EDAT- 2001/03/07 10:00
MHDA- 2001/03/27 10:01
CRDT- 2001/03/07 10:00
PHST- 2001/03/07 10:00 [pubmed]
PHST- 2001/03/27 10:01 [medline]
PHST- 2001/03/07 10:00 [entrez]
AID - 10.1002/1529-0131(200102)44:2<343::AID-ANR52>3.0.CO;2-Q [doi]
PST - ppublish
SO  - Arthritis Rheum. 2001 Feb;44(2):343-50. doi: 
      10.1002/1529-0131(200102)44:2<343::AID-ANR52>3.0.CO;2-Q.