PMID- 11230411
OWN - NLM
STAT- MEDLINE
DCOM- 20010719
LR  - 20220316
IS  - 0095-1137 (Print)
IS  - 1098-660X (Electronic)
IS  - 0095-1137 (Linking)
VI  - 39
IP  - 3
DP  - 2001 Mar
TI  - Use of immunoglobulin G antibody avidity for differentiation of primary human 
      herpesvirus 6 and 7 infections.
PG  - 959-63
AB  - A human herpesvirus 7 (HHV-7) indirect immunofluorescence antibody avidity test 
      was developed and used with an existing human herpesvirus 6 (HHV-6) antibody 
      avidity test to detect and distinguish low-avidity antibodies to HHV-6 and HHV-7 
      and hence the respective primary infections. With sera from 269 British children 
      aged 0 to 179 weeks, the tests showed that most (10 of 98 serum samples [13%]) 
      HHV-6 low-avidity antibody was found in the first year of life, whereas for 
      HHV-7, most (18 of 101 serum samples [20%]) HHV-7 low-avidity antibody was found 
      in the second year of life. Five children had low-avidity antibodies to both 
      viruses. Of nine Japanese children with previously serologically proven primary 
      HHV-6 or HHV-7 infections, eight had low-avidity antibody only to the relevant 
      virus, but one child had low-avidity antibodies to HHV-6 and HHV-7. The avidity 
      tests were applied to five British children and further proof of viral infection 
      was sought by the detection of specific DNA in serum or plasma, and saliva or 
      cerebrospinal fluid. In two children who had low-avidity antibody to HHV-7 but 
      who were seronegative for HHV-6, only HHV-7 was found. Both viruses were detected 
      in one child with low-avidity HHV-7 antibody and high-avidity HHV-6 antibody. In 
      two children with low-avidity antibodies to both viruses, HHV-6 and HHV-7 DNAs 
      were found, confirming dual primary infections and excluding antibody 
      cross-reactivity.
FAU - Ward, K N
AU  - Ward KN
AD  - Department of Infectious Diseases, Imperial College School of Medicine, 
      Hammersmith Campus, London W12 ONN, United Kingdom. k.n.ward@ucl.ac.uk
FAU - Turner, D J
AU  - Turner DJ
FAU - Parada, X C
AU  - Parada XC
FAU - Thiruchelvam, A D
AU  - Thiruchelvam AD
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Clin Microbiol
JT  - Journal of clinical microbiology
JID - 7505564
RN  - 0 (Antibodies, Viral)
RN  - 0 (DNA, Viral)
RN  - 0 (Immunoglobulin G)
SB  - IM
MH  - Adult
MH  - Antibodies, Viral/blood/immunology
MH  - *Antibody Affinity
MH  - Child, Preschool
MH  - DNA, Viral/blood
MH  - Female
MH  - Herpesviridae Infections/*diagnosis/virology
MH  - Herpesvirus 6, Human/*immunology
MH  - Herpesvirus 7, Human/*immunology
MH  - Humans
MH  - Immunoglobulin G/blood/*immunology
MH  - Infant
MH  - Infant, Newborn
MH  - Male
MH  - Polymerase Chain Reaction
MH  - Species Specificity
PMC - PMC87857
EDAT- 2001/03/07 10:00
MHDA- 2001/07/20 10:01
PMCR- 2001/03/01
CRDT- 2001/03/07 10:00
PHST- 2001/03/07 10:00 [pubmed]
PHST- 2001/07/20 10:01 [medline]
PHST- 2001/03/07 10:00 [entrez]
PHST- 2001/03/01 00:00 [pmc-release]
AID - 1053 [pii]
AID - 10.1128/JCM.39.3.959-963.2001 [doi]
PST - ppublish
SO  - J Clin Microbiol. 2001 Mar;39(3):959-63. doi: 10.1128/JCM.39.3.959-963.2001.