PMID- 11230557
OWN - NLM
STAT- MEDLINE
DCOM- 20010607
LR  - 20190513
IS  - 0267-8357 (Print)
IS  - 0267-8357 (Linking)
VI  - 16
IP  - 2
DP  - 2001 Mar
TI  - Chromosomal composition of micronuclei in mouse NIH 3T3 cells treated with 
      acrylamide, extract of Tripterygium hypoglaucum (level) hutch, mitomycin C and 
      colchicine, detected by multicolor FISH with centromeric and telomeric DNA 
      probes.
PG  - 145-9
AB  - The chromosomal composition of micronuclei (MN) induced by the model mutagens 
      mitomycin (MMC) and colchicine (COL) as well as by acrylamide (AA) and the 
      traditional Chinese medicine Tripterygium hypoglaucum (level) hutch (THH) in NIH 
      3T3 cells was analyzed by multicolor fluorescence in situ hybridization (FISH) 
      using DNA probes for the centromere repeated minor satellite DNA and the 
      telomeric hexamer repeat (TTAGGG). The majority of MN (78.6%) from treatment with 
      MMC (0.1 microg/ml) did not show centromeric signals, reflecting the clastogenic 
      action of MMC. Following treatment with COL (0.1 microg/ml), 74.5% of the MN 
      showed centromeric signals and several telomeric signals, indicating that MN 
      induced by this well-known aneugen were mainly composed of whole chromosomes. 
      After treatment with AA (100, 200 and 400 microg/ml) both MN containing whole 
      chromosomes and MN containing acentric fragments were found to increase in a 
      dose-dependent manner, demonstrating that AA is not only a clastogen but also an 
      aneugen. THH induced a high frequency of MN harboring whole chromosomes at all 
      concentrations tested (5, 10 and 20 microl/ml) and produced a dose-dependent 
      increase in fragment-containing MN, indicating that THH has both aneugenic and 
      clastogenic potential.
FAU - Jie, Y M
AU  - Jie YM
AD  - Molecular Toxicology Laboratory, Third Military Medical University, Chongqing 
      400038, China.
FAU - Jia, C
AU  - Jia C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Mutagenesis
JT  - Mutagenesis
JID - 8707812
RN  - 0 (Alkylating Agents)
RN  - 0 (Antineoplastic Agents, Phytogenic)
RN  - 0 (DNA Probes)
RN  - 0 (Drugs, Chinese Herbal)
RN  - 0 (Mutagens)
RN  - 20R035KLCI (Acrylamide)
RN  - 50SG953SK6 (Mitomycin)
RN  - SML2Y3J35T (Colchicine)
SB  - IM
MH  - 3T3 Cells
MH  - Acrylamide/*pharmacology
MH  - Alkylating Agents/pharmacology
MH  - Animals
MH  - Antineoplastic Agents, Phytogenic/pharmacology
MH  - Centromere/drug effects/genetics
MH  - Chromosomes/*drug effects
MH  - Colchicine/*pharmacology
MH  - DNA Probes/*genetics
MH  - Drugs, Chinese Herbal/*pharmacology
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Mice
MH  - Micronuclei, Chromosome-Defective/*drug effects/genetics
MH  - Mitomycin/*pharmacology
MH  - Mutagens/pharmacology
MH  - Telomere/drug effects/genetics
MH  - Tripterygium
EDAT- 2001/03/07 10:00
MHDA- 2001/06/08 10:01
CRDT- 2001/03/07 10:00
PHST- 2001/03/07 10:00 [pubmed]
PHST- 2001/06/08 10:01 [medline]
PHST- 2001/03/07 10:00 [entrez]
AID - 10.1093/mutage/16.2.145 [doi]
PST - ppublish
SO  - Mutagenesis. 2001 Mar;16(2):145-9. doi: 10.1093/mutage/16.2.145.