PMID- 11232040
OWN - NLM
STAT- MEDLINE
DCOM- 20010412
LR  - 20111117
IS  - 0021-972X (Print)
IS  - 0021-972X (Linking)
VI  - 86
IP  - 1
DP  - 2001 Jan
TI  - Insulin inhibits NFkappaB and MCP-1 expression in human aortic endothelial cells.
PG  - 450-3
AB  - In view of our recent demonstration that insulin inhibits the expression of 
      intercellular adhesion molecule-1 (ICAM-1) and the fact that ICAM-1 expression is 
      known to be modulated by nuclear factor-kappaB (NFkappaB), we have now 
      investigated whether insulin inhibits intranuclear NFkappaB binding activity. We 
      have also investigated whether insulin inhibits the pro-inflammatory chemokine, 
      monocyte chemoattractant protein-1 (MCP-1), which attracts leucocytes to the 
      inflamed sites and is also regulated by NFkappaB. Insulin was incubated with 
      cultured human aortic endothelial cells (HAEC) at 0, 100 and 1000 microU/mL. 
      Intranuclear NFkappaB binding activity was suppressed by approximately 45% at 100 
      microU/mL and by 60% at 1000 microU/mL (p < 0.05). MCP-1 mRNA expression was also 
      suppressed by 47% at 100 microU/mL and by 79% at 1000 microU/mL (p < 0.05). We 
      conclude that insulin at physiologically relevant concentrations exerts an 
      inhibitory effect on the cardinal pro-inflammatory transcription factor NFkappaB 
      and the pro-inflammatory chemokine MCP-1; these effects suggest an 
      anti-inflammatory and potential anti-atherogenic effects of insulin.
FAU - Aljada, A
AU  - Aljada A
AD  - Division of Endocrinology, Diabetes and Metabolism, State University of New York 
      at Buffalo and Kaleida Health, Buffalo, New York 14209, USA.
FAU - Ghanim, H
AU  - Ghanim H
FAU - Saadeh, R
AU  - Saadeh R
FAU - Dandona, P
AU  - Dandona P
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Clin Endocrinol Metab
JT  - The Journal of clinical endocrinology and metabolism
JID - 0375362
RN  - 0 (Chemokine CCL2)
RN  - 0 (Insulin)
RN  - 0 (NF-kappa B)
SB  - IM
MH  - Aorta/cytology/*metabolism
MH  - Cells, Cultured
MH  - Chemokine CCL2/*antagonists & inhibitors
MH  - Endothelium, Vascular/cytology/*metabolism
MH  - Humans
MH  - Insulin/*pharmacology
MH  - NF-kappa B/*antagonists & inhibitors
EDAT- 2001/03/07 10:00
MHDA- 2001/04/17 10:01
CRDT- 2001/03/07 10:00
PHST- 2001/03/07 10:00 [pubmed]
PHST- 2001/04/17 10:01 [medline]
PHST- 2001/03/07 10:00 [entrez]
AID - 10.1210/jcem.86.1.7278 [doi]
PST - ppublish
SO  - J Clin Endocrinol Metab. 2001 Jan;86(1):450-3. doi: 10.1210/jcem.86.1.7278.