PMID- 11232698
OWN - NLM
STAT- MEDLINE
DCOM- 20010329
LR  - 20101118
IS  - 0002-9270 (Print)
IS  - 0002-9270 (Linking)
VI  - 96
IP  - 2
DP  - 2001 Feb
TI  - Immunoglobulin E and eosinophilic cationic protein in segmental lavage fluid of 
      the small and large bowel identify patients with food allergy.
PG  - 508-14
AB  - OBJECTIVE: Members of the general population often assume that they suffer from 
      food allergy, but the true prevalence is low. Testing for the diagnosis of 
      food-related hypersensitivity entails laborious procedures, including GI 
      endoscopy. Our objective was to develop an endoscopic screening approach for food 
      allergy. METHODS: Endoscopically guided segmental lavage was performed in 11 
      patients with GI allergy and in 20 controls during lower GI endoscopy of the 
      terminal ileum, the coecum, and the rectosigmoid. Eosinophilic cationic protein 
      (ECP) and protein were measured in native lavage fluid, and immunoglobulin E 
      (IgE) was also measured after a 10-fold lavage concentration. RESULTS: 
      IgE/protein in lavage fluid from the coecum (0.055 +/- 0.068 U/mg vs 0.003 +/- 
      0.012 U/mg; p = 0.001) and the rectosigmoid (0.134 +/- 0.170 U/mg vs 0.019 +/- 
      0.042 U/mg; p < 0.05) was significantly elevated in patients with GI allergy. 
      ECP/protein was significantly elevated at the terminal ileum (22.95 +/- 37.67 
      microg/mg vs 7.09 +/- 7.68 microg/mg; p < 0.05) and the rectosigmoid (23.66 +/- 
      19.43 microg/mg vs 11.97 +/- 16.39 microg/mg; p < 0.05). The combined use of GI 
      lavage IgE and ECP as a diagnostic test for food allergy resulted in a 
      sensitivity of 91% and a specificity of 80%. CONCLUSIONS: In endoscopically 
      guided segmental lavage fluid, IgE and ECP/protein are increased in patients with 
      food allergy. These measurements seem to offer an attractive diagnostic tool and 
      may serve as a screening method.
FAU - Schwab, D
AU  - Schwab D
AD  - Department of Medicine I, Erlangen, Germany.
FAU - Raithel, M
AU  - Raithel M
FAU - Klein, P
AU  - Klein P
FAU - Winterkamp, S
AU  - Winterkamp S
FAU - Weidenhiller, M
AU  - Weidenhiller M
FAU - Radespiel-Troeger, M
AU  - Radespiel-Troeger M
FAU - Hochberger, J
AU  - Hochberger J
FAU - Hahn, E G
AU  - Hahn EG
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Am J Gastroenterol
JT  - The American journal of gastroenterology
JID - 0421030
RN  - 0 (Blood Proteins)
RN  - 0 (Eosinophil Granule Proteins)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - EC 3.1.- (Ribonucleases)
SB  - IM
MH  - Adult
MH  - Blood Proteins/*analysis
MH  - Case-Control Studies
MH  - Double-Blind Method
MH  - Endoscopy, Gastrointestinal
MH  - Eosinophil Granule Proteins
MH  - Eosinophils/metabolism
MH  - Female
MH  - Food Hypersensitivity/*diagnosis/immunology
MH  - Humans
MH  - Ileum/metabolism
MH  - Immunoglobulin E/*analysis
MH  - Male
MH  - Middle Aged
MH  - Radioallergosorbent Test
MH  - *Ribonucleases
MH  - Sensitivity and Specificity
MH  - Skin Tests
MH  - Therapeutic Irrigation
EDAT- 2001/03/10 10:00
MHDA- 2001/04/03 10:01
CRDT- 2001/03/10 10:00
PHST- 2001/03/10 10:00 [pubmed]
PHST- 2001/04/03 10:01 [medline]
PHST- 2001/03/10 10:00 [entrez]
AID - S0002-9270(00)02260-7 [pii]
AID - 10.1111/j.1572-0241.2001.03467.x [doi]
PST - ppublish
SO  - Am J Gastroenterol. 2001 Feb;96(2):508-14. doi: 10.1111/j.1572-0241.2001.03467.x.