PMID- 11234927
OWN - NLM
STAT- MEDLINE
DCOM- 20010621
LR  - 20191104
IS  - 1462-2912 (Print)
IS  - 1462-2912 (Linking)
VI  - 2
IP  - 4
DP  - 2000 Aug
TI  - Studies on the in situ physiology of Thiothrix spp. present in activated sludge.
PG  - 389-98
AB  - The in situ physiology of the filamentous sulphur bacterium Thiothrix spp. was 
      investigated in an industrial wastewater treatment plant with severe bulking 
      problems as a result of overgrowth of Thiothrix. Identification and enumeration 
      using fluorescence in situ hybridization (FISH) with species-specific 16S and 23S 
      rRNA probes revealed that 5-10% of the bacteria in the activated sludge were 
      Thiothrix spp. By using a combination of FISH and microautoradiography it was 
      possible to study the in situ physiology of probe-defined Thiothrix filaments 
      under different environmental conditions. The Thiothrix filaments were very 
      versatile and showed incorporation of radiolabelled acetate and/or bicarbonate 
      under heterotrophic, mixotrophic and chemolithoautotrophic conditions. The 
      Thiothrix filaments were active under anaerobic conditions (with or without 
      nitrate) in which intracellular sulphur globules were formed from thiosulphate 
      and acetate was taken up. Thiothrix-specific substrate uptake rates and growth 
      rates in activated sludge samples were determined under different conditions. 
      Doubling times of 6-9 h under mixotrophic conditions and 15-30 h under 
      autotrophic conditions were estimated. The key properties that Thiothrix might be 
      employing to outcompete other microorganisms in activated sludge were probably 
      related to the mixotrophic growth potential with strong stimulation of acetate 
      uptake by thiosulphate, as well as stimulation of bicarbonate incorporation by 
      acetate in the presence of thiosulphate.
FAU - Nielsen, P H
AU  - Nielsen PH
AD  - Environmental Engineering Laboratory, Aalborg University, Denmark. 
      i5phn@civil.auc.dk
FAU - de Muro, M A
AU  - de Muro MA
FAU - Nielsen, J L
AU  - Nielsen JL
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Environ Microbiol
JT  - Environmental microbiology
JID - 100883692
RN  - 0 (Acetates)
RN  - 0 (Bicarbonates)
RN  - 0 (Carbon Radioisotopes)
RN  - 0 (Sewage)
RN  - 0 (Thiosulfates)
RN  - 10028-17-8 (Tritium)
RN  - 70FD1KFU70 (Sulfur)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - Acetates/metabolism
MH  - Anaerobiosis
MH  - Bicarbonates/metabolism
MH  - Carbon Radioisotopes/metabolism
MH  - Gammaproteobacteria/classification/genetics/isolation & purification/*metabolism
MH  - Glucose/metabolism
MH  - In Situ Hybridization, Fluorescence
MH  - Microscopy, Confocal
MH  - Sewage/*microbiology
MH  - Sulfur/metabolism
MH  - Thiosulfates/metabolism
MH  - Tritium/metabolism
MH  - Waste Disposal, Fluid
EDAT- 2001/03/10 10:00
MHDA- 2001/06/22 10:01
CRDT- 2001/03/10 10:00
PHST- 2001/03/10 10:00 [pubmed]
PHST- 2001/06/22 10:01 [medline]
PHST- 2001/03/10 10:00 [entrez]
AID - 10.1046/j.1462-2920.2000.00120.x [doi]
PST - ppublish
SO  - Environ Microbiol. 2000 Aug;2(4):389-98. doi: 10.1046/j.1462-2920.2000.00120.x.