PMID- 11235096
OWN - NLM
STAT- MEDLINE
DCOM- 20010329
LR  - 20230303
IS  - 0003-2654 (Print)
IS  - 0003-2654 (Linking)
VI  - 126
IP  - 2
DP  - 2001 Feb
TI  - Multi-residue analysis of avermectins and moxidectin by ion-trap LC-MSn.
PG  - 155-60
AB  - A multi-residue method was developed and validated for the quantitation and 
      confirmation of avermectins and moxidectin residues in bovine liver. Target 
      analytes were extracted from liver homogenate using C8 solid phase cartridges, 
      chromatographed under basic pH conditions in order to promote the formation of 
      analyte anions, and detected by ion-trap mass spectrometry (MS) in negative ion 
      mode using an atmospheric pressure chemical ionization interface (APCI). The 
      method provided detection capabilities (CC beta, where beta = 0.05) for 
      eprinomectin, abamectin, doramectin, moxidectin and ivermectin of 3.1, 3.2, 2.2, 
      4.0 and 3.2 ng g-1 liver respectively, well below their respective maximum 
      residue limits (MRLs). The critical concentrations for MRL compliance (CC alpha, 
      where alpha = 0.01) were 840, 28, 130, 130 and 130 ng g-1 respectively. Analysis 
      of liver fortified at the appropriate MRLs gave recoveries (% +/- RSD) of 70.9 
      +/- 11.6 (n = 14), 69.1 +/- 3.9 (n = 13), 65.9 +/- 6.4 (n = 19), 69.7 +/- 9.3 (n 
      = 19) and 73.2 +/- 10.5 (n = 19), respectively, for each analyte. Calibration 
      curves fitted a second order polynomial function (R2 > or = 0.9978) over a wide 
      range of concentrations (0 to 10,000 ng ml-1). The detection of two daughter-ions 
      for each analyte allowed for quantitation and the confirmation of identity. The 
      method is suitable for application in European Union statutory veterinary drug 
      residue surveillance programmes, since it fulfills appropriate analytical 
      criteria, and has the particular advantage of enabling high throughput 
      multi-residue quantitation and confirmation of the target analytes.
FAU - Howells, L
AU  - Howells L
AD  - Department of Risk Research, Veterinary Laboratories Agency, Addlestone, Surrey, 
      UK KT15 3NB.
FAU - Sauer, M J
AU  - Sauer MJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Validation Study
PL  - England
TA  - Analyst
JT  - The Analyst
JID - 0372652
RN  - 0 (Anthelmintics)
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (Insecticides)
RN  - 0 (Macrolides)
RN  - 70288-86-7 (Ivermectin)
RN  - 73989-17-0 (avermectin)
RN  - NGU5H31YO9 (moxidectin)
SB  - IM
MH  - Animals
MH  - Anthelmintics/*analysis
MH  - Anti-Bacterial Agents/*analysis
MH  - Cattle
MH  - *Drug Residues
MH  - Insecticides/*analysis
MH  - Ivermectin/*analogs & derivatives/*analysis
MH  - Liver/chemistry
MH  - Macrolides
MH  - Mass Spectrometry/methods
MH  - Meat/analysis
EDAT- 2001/03/10 10:00
MHDA- 2001/04/03 10:01
CRDT- 2001/03/10 10:00
PHST- 2001/03/10 10:00 [pubmed]
PHST- 2001/04/03 10:01 [medline]
PHST- 2001/03/10 10:00 [entrez]
AID - 10.1039/b008305o [doi]
PST - ppublish
SO  - Analyst. 2001 Feb;126(2):155-60. doi: 10.1039/b008305o.