PMID- 11235568
OWN - NLM
STAT- MEDLINE
DCOM- 20021017
LR  - 20061115
IS  - 0253-3766 (Print)
IS  - 0253-3766 (Linking)
VI  - 22
IP  - 6
DP  - 2000 Nov
TI  - [Application of fluorescence in situ hybridization (FISH) in sputum cytologic 
      diagnosis of lung cancer].
PG  - 477-9
AB  - OBJECTIVE: To study the numerical chromosomal abnormalities of cells in sputum 
      from patients with lung cancer by dual-fluorescence in situ hybridization (FISH). 
      METHODS: Thirty sputum samples from lung cancer patients were examined by FISH 
      with centromere DNA probes of chromosome 7, 11, 17 and X. RESULTS: In 23 positive 
      sputum samples studied, the frequency of hyperdiploid of chromosome 7, 17, X and 
      11 was 65.2% (15/23), 60.9% (14/23), 52.2% (12/23) and 39.1% (9/23), 
      respectively. Hyperdiploidy of chromosome 7 was found in 4 of 7 sputum samples 
      which were cytologicaly suspicious of cancer cells. CONCLUSION: FISH can detect 
      aneuploid malignant cells in sputum and be used as a complementary technique to 
      cytologic diagnosis of lung cancer.
FAU - Jia, D
AU  - Jia D
AD  - Cancer Institute (Hospital), Chinese Academy of Medical Sciences, Peking Union 
      Medical College, Beijing 100021, China.
FAU - Zhang, Z
AU  - Zhang Z
FAU - Liu, S
AU  - Liu S
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhonghua Zhong Liu Za Zhi
JT  - Zhonghua zhong liu za zhi [Chinese journal of oncology]
JID - 7910681
SB  - IM
MH  - Adenocarcinoma/*diagnosis/genetics
MH  - Carcinoma, Squamous Cell/*diagnosis/genetics
MH  - Diploidy
MH  - Female
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Lung Neoplasms/*diagnosis/genetics
MH  - Male
MH  - Sputum/*cytology
EDAT- 2001/03/10 10:00
MHDA- 2002/10/18 04:00
CRDT- 2001/03/10 10:00
PHST- 2001/03/10 10:00 [pubmed]
PHST- 2002/10/18 04:00 [medline]
PHST- 2001/03/10 10:00 [entrez]
PST - ppublish
SO  - Zhonghua Zhong Liu Za Zhi. 2000 Nov;22(6):477-9.