PMID- 11236003
OWN - NLM
STAT- MEDLINE
DCOM- 20010329
LR  - 20190720
IS  - 0302-766X (Print)
IS  - 0302-766X (Linking)
VI  - 303
IP  - 1
DP  - 2001 Jan
TI  - Distinctive spatiotemporal expression patterns for neurotrophins develop in 
      gustatory papillae and lingual tissues in embryonic tongue organ cultures.
PG  - 35-45
AB  - Brain-derived neurotrophic factor (BDNF) and neurotrophin-3 (NT-3) mRNAs are 
      expressed in the developing rat tongue and taste organs in specific 
      spatiotemporal patterns. BDNF mRNA is present in the early lingual gustatory 
      papilla epithelium, from which taste buds eventually arise, prior to the arrival 
      of gustatory nerve fibers at the epithelium, whereas NT-3 initially distributes 
      in the mesenchyme. However, a direct test for neural dependence of neurotrophin 
      expression on the presence of innervation in tongue has not been made, nor is it 
      known whether the patterns of neurotrophin expression can be replicated in an in 
      vitro system. Therefore, we used a tongue organ culture model that supports taste 
      papilla formation while eliminating the influence from sensory nerve fibers, to 
      study neurotrophin mRNAs in lingual tissues. Rat tongue cultures were begun at 
      embryonic day 13 or 14 (E13, E14), and BDNF, NT-3, nerve growth factor (NGF) and 
      neurotrophin-4 (NT-4) mRNAs were studied at 0, 2, 3 and 6 days in culture. BDNF 
      transcripts were localized in the gustatory epithelium of both developing 
      fungiform and circumvallate papillae after 2 or 3 days in culture, and NT-3 
      transcripts were in the subepithelial mesenchyme. The neurotrophin distributions 
      were comparable to those in vivo at E13-E16. In 6-day tongue cultures, however, 
      BDNF transcripts in anterior tongue were not restricted to fungiform papillae but 
      were more widespread in the lingual epithelium, while the circumvallate trench 
      epithelium exhibited restricted BDNF labeling. The NT-3 expression pattern 
      shifted in 6-day organ cultures in a manner comparable to that in the embryo in 
      vivo, and was expressed in the lingual epithelium as well as mesenchyme. NGF mRNA 
      expression was subepithelial throughout 6 days in cultures. NT-4 mRNA was not 
      detected. The neurotrophin mRNA distributions demonstrate that temporospatial 
      localization of neurotrophins observed during development in vivo is retained in 
      the embryonic tongue organ culture system. Furthermore, initial neurotrophin 
      expression in the developing lingual epithelium, mesenchyme, and/or taste 
      papillae is not dependent on intact sensory innervation. We suggest that patterns 
      of lingual neurotrophin mRNA expression are controlled by the influence of local 
      tissue interactions within the tongue at early developmental stages. However, the 
      eventual loss of restricted BDNF mRNA localization from fungiform papillae in 
      anterior tongue suggests that sensory innervation may be important for 
      restricting the localized expression of neurotrophins at later developmental 
      stages, and for maintaining the unique phenotypes of gustatory papillae.
FAU - Nosrat, C A
AU  - Nosrat CA
AD  - Department of Neuroscience, Karolinska Institutet, Stockholm, Sweden. 
      nosrat@umich.edu
FAU - MacCallum, D K
AU  - MacCallum DK
FAU - Mistretta, C M
AU  - Mistretta CM
LA  - eng
GR  - R01 DC000456/DC/NIDCD NIH HHS/United States
GR  - DC00456/DC/NIDCD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Germany
TA  - Cell Tissue Res
JT  - Cell and tissue research
JID - 0417625
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Nerve Growth Factors)
RN  - 0 (Neurotrophin 3)
RN  - 0 (RNA, Messenger)
RN  - 9061-61-4 (Nerve Growth Factor)
RN  - P658DCA9XD (neurotrophin 4)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/analysis/genetics
MH  - Female
MH  - Gene Expression Regulation, Developmental
MH  - In Situ Hybridization
MH  - Lingual Nerve/embryology
MH  - Mesoderm/physiology/ultrastructure
MH  - Nerve Growth Factor/analysis/genetics
MH  - Nerve Growth Factors/analysis/*genetics
MH  - Neurotrophin 3/analysis/genetics
MH  - Organ Culture Techniques
MH  - Pregnancy
MH  - RNA, Messenger/analysis
MH  - Rats
MH  - Taste/physiology
MH  - Taste Buds/*embryology/*physiology/ultrastructure
MH  - Tongue/embryology/*innervation/ultrastructure
EDAT- 2001/03/10 10:00
MHDA- 2001/04/03 10:01
CRDT- 2001/03/10 10:00
PHST- 2001/03/10 10:00 [pubmed]
PHST- 2001/04/03 10:01 [medline]
PHST- 2001/03/10 10:00 [entrez]
AID - 10.1007/s004410000271 [doi]
PST - ppublish
SO  - Cell Tissue Res. 2001 Jan;303(1):35-45. doi: 10.1007/s004410000271.