PMID- 11236899
OWN - NLM
STAT- MEDLINE
DCOM- 20010607
LR  - 20190915
IS  - 1073-2322 (Print)
IS  - 1073-2322 (Linking)
VI  - 15
IP  - 3
DP  - 2001 Mar
TI  - Chemokines and interleukin-18 are up-regulated in bronchoalveolar lavage fluid 
      but not in serum of septic surgical ICU patients.
PG  - 176-80
AB  - Our objective was to investigate the levels of chemokines (MIP1-alpha, MCP-1, and 
      Gro-alpha), Interleukin-18 (IL-18), and Interleukin (IL-6) in bronchoalveolar 
      lavage (BAL) fluid and serum at the onset and ongoing states of sepsis as defined 
      by the American College of Chest Physicians/Society of Critical Care Medicine in 
      septic surgical ICU patients. Our summary background data was to understand the 
      significance of compartmentalized inflammatory mediator production in an 
      immunologically active organ (lung) in comparison with levels in the systemic 
      circulation. The study group consisted of 20 septic patients and 10 non-septic 
      patients on surgical ICU. At the onset of sepsis, both BAL fluid and serum 
      samples were taken and levels of MIP-1alpha, MCP-1, GRO-alpha, IL-18, and IL-6 
      were measured by ELISA. Furthermore, over a subsequent 8-day period, levels of 
      these mediators were determined in serum. In some experiments, IL-18 mRNA levels 
      were determined in peripheral blood lymphocytes (PBL) of septic and non-septic 
      patients. At the onset of sepsis, MIP-1alpha, MCP-1, GRO-alpha, IL-18, and IL-6 
      levels were significantly up-regulated in BAL fluid as compared with non-septic 
      controls. In marked contrast, with the exception of IL-18 mRNA and IL-6 peptide, 
      there was no increase in serum levels of inflammatory mediators determined both 
      at the onset and during the ongoing states of sepsis. Based on the present data, 
      monitoring levels of serum chemokines and IL-18 protein as markers of sepsis 
      might be misleading since despite their non-detection in serum, they were highly 
      up-regulated in the lung tissue compartment. These data might underscore the role 
      of MIP-1alpha, MCP-1, GRO-alpha, and IL-18 in the mediation of local tissue 
      damage. Furthermore, these findings raise the notion that mediator measurement in 
      immunologically active organs might serve as pivotal indicators of sepsis prior 
      to the actual fulfillment of specific clinical criteria that defines the patient 
      as being septic.
FAU - Mathiak, G
AU  - Mathiak G
AD  - Department of Surgery, University of Cologne, Germany.
FAU - Neville, L F
AU  - Neville LF
FAU - Grass, G
AU  - Grass G
FAU - Boehm, S A
AU  - Boehm SA
FAU - Luebke, T
AU  - Luebke T
FAU - Herzmann, T
AU  - Herzmann T
FAU - Kabir, K
AU  - Kabir K
FAU - Rosendahl, R
AU  - Rosendahl R
FAU - Schaefer, U
AU  - Schaefer U
FAU - Mueller, C
AU  - Mueller C
FAU - Bohlen, H
AU  - Bohlen H
FAU - Wassermann, K
AU  - Wassermann K
FAU - Hoelscher, A H
AU  - Hoelscher AH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Shock
JT  - Shock (Augusta, Ga.)
JID - 9421564
RN  - 0 (Chemokines)
RN  - 0 (Interleukin-18)
RN  - 0 (Interleukin-6)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - *Bronchoalveolar Lavage Fluid
MH  - Case-Control Studies
MH  - Chemokines/blood/genetics/*metabolism
MH  - *Critical Care
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Humans
MH  - Interleukin-18/blood/genetics/*metabolism
MH  - Interleukin-6/metabolism
MH  - Postoperative Complications
MH  - RNA, Messenger/metabolism
MH  - Sepsis/*metabolism
MH  - Up-Regulation
EDAT- 2001/03/10 10:00
MHDA- 2001/06/19 10:01
CRDT- 2001/03/10 10:00
PHST- 2001/03/10 10:00 [pubmed]
PHST- 2001/06/19 10:01 [medline]
PHST- 2001/03/10 10:00 [entrez]
AID - 10.1097/00024382-200115030-00003 [doi]
PST - ppublish
SO  - Shock. 2001 Mar;15(3):176-80. doi: 10.1097/00024382-200115030-00003.