PMID- 11237250
OWN - NLM
STAT- MEDLINE
DCOM- 20010614
LR  - 20190910
IS  - 0162-0134 (Print)
IS  - 0162-0134 (Linking)
VI  - 83
IP  - 2-3
DP  - 2001 Jan 15
TI  - The EPR spectrum for CuB in cytochrome c oxidase.
PG  - 115-9
AB  - Incubation of cytochrome c oxidase (CcO) in its resting state in saturated 
      ammonium sulfate, at room temperature overnight, gave EPR signals characteristic 
      of a single Cu(II) center. From the g// and A// values it is concluded that this 
      is a square-planar type 2 copper center, and superhyperfine splitting shows the 
      presence of three nearly equivalent 14N nuclei in the plane. It is suggested that 
      this center, also formed by incubating the enzyme in 10% methanol followed by 
      direct irradiation, must be the CuB center. This type 2 copper EPR spectrum is 
      identical to the EPR spectrum of CuB reported for the isolated cytochrome bo3 
      complex from Escherichia coli; and to the EPR spectrum reported for the 
      sulfobetaine 12 heat-treated cytochrome c oxidase complex. It is argued that a 
      small perturbation in the system causes decoupling of the magnetic coupling of 
      the heme a3-CuB binuclear center and the appearance of the type 2 EPR signal.
FAU - Pezeshk, A
AU  - Pezeshk A
AD  - Department of Chemistry, Minnesota State University Moorhead 56563, USA. 
      pezeshk@mnstate.edu
FAU - Torres, J
AU  - Torres J
FAU - Wilson, M T
AU  - Wilson MT
FAU - Symons, M C
AU  - Symons MC
LA  - eng
GR  - R15CA74356/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Inorg Biochem
JT  - Journal of inorganic biochemistry
JID - 7905788
RN  - 789U1901C5 (Copper)
RN  - EC 1.9.3.1 (Electron Transport Complex IV)
RN  - SU46BAM238 (Ammonium Sulfate)
SB  - IM
MH  - Ammonium Sulfate/chemistry
MH  - Copper/*chemistry
MH  - *Electron Spin Resonance Spectroscopy
MH  - Electron Transport Complex IV/*chemistry/radiation effects
MH  - Gamma Rays
MH  - Oxidation-Reduction
EDAT- 2001/03/10 10:00
MHDA- 2001/06/15 10:01
CRDT- 2001/03/10 10:00
PHST- 2001/03/10 10:00 [pubmed]
PHST- 2001/06/15 10:01 [medline]
PHST- 2001/03/10 10:00 [entrez]
AID - S0162-0134(00)00189-6 [pii]
AID - 10.1016/s0162-0134(00)00189-6 [doi]
PST - ppublish
SO  - J Inorg Biochem. 2001 Jan 15;83(2-3):115-9. doi: 10.1016/s0162-0134(00)00189-6.