PMID- 11237661
OWN - NLM
STAT- MEDLINE
DCOM- 20010517
LR  - 20161018
IS  - 1063-4584 (Print)
IS  - 1063-4584 (Linking)
VI  - 9
IP  - 2
DP  - 2001 Feb
TI  - Acridine orange induces binucleation in chondrocytes.
PG  - 147-51
AB  - OBJECTIVE: Although it is well known that binuclear cells commonly appear among 
      the chondrocytes of normal cartilages as well as among neoplastic chondrocytes of 
      chondrosarcomas, the mechanism of binucleation is still unclear. Therefore, this 
      study was undertaken to clarify the mechanism of binucleation in chondrocytes, 
      using primary culture cells of growth plate cartilage. DESIGN: These chondrocytes 
      were exposed to acridine orange (AO) which is a fluorescent dye for 
      differentiating certain DNAs and RNAs in nuclei and cytoplasm, and which inhibits 
      mitosis. After exposure to 0.5 microg/ml AO, for 0, 6, 24, 48, and 96 h, the 
      following parameters were investigated: (1) cell growth rate (GR); (2) frequency 
      of hyperdiploid cells (%HDC) by DNA cytofluorometry; (3) mitotic index (MI); (4) 
      BrdU labeling index (LI); (5) frequency of binuclear cells (%BNC). RESULTS: 
      Compared with the control cells, which were cultured in AO-free medium, the GR 
      was remarkably inhibited at 24 h. MI was also decreased from 6 to 24 h, and LI 
      decreased at 48 h. However, these parameters were recovered at 96 h. The %HDC was 
      increased from 6 to 96 h, and the %BNC was also increased to a maximum of six 
      times that of the control cells at 96 h. DISCUSSION: These results suggested that 
      the binuclear cells observed among the cultured chondrocytes may be formed from 
      G2 arrested cells by amitotic nuclear division, but not by mitosis without 
      cytoplasmic division or cell fusion.
CI  - Copyright 2001
FAU - Kusuzaki, K
AU  - Kusuzaki K
AD  - Department of Orthopaedic Surgery, Kyoto Prefectural University of Medicine, 
      Japan. kusu@basic.kpu-m.ac.jp
FAU - Takeshita, H
AU  - Takeshita H
FAU - Murata, H
AU  - Murata H
FAU - Hashiguchi, S
AU  - Hashiguchi S
FAU - Nozaki, T
AU  - Nozaki T
FAU - Emoto, K
AU  - Emoto K
FAU - Ashihara, T
AU  - Ashihara T
FAU - Hirasawa, Y
AU  - Hirasawa Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Osteoarthritis Cartilage
JT  - Osteoarthritis and cartilage
JID - 9305697
RN  - 9007-49-2 (DNA)
RN  - F30N4O6XVV (Acridine Orange)
SB  - IM
MH  - Acridine Orange/*pharmacology
MH  - Animals
MH  - Cattle
MH  - Cell Division/drug effects
MH  - Chondrocytes/*cytology
MH  - DNA/metabolism
MH  - Mitosis/drug effects
EDAT- 2001/03/10 10:00
MHDA- 2001/05/18 10:01
CRDT- 2001/03/10 10:00
PHST- 2001/03/10 10:00 [pubmed]
PHST- 2001/05/18 10:01 [medline]
PHST- 2001/03/10 10:00 [entrez]
AID - S1063-4584(00)90371-6 [pii]
AID - 10.1053/joca.2000.0371 [doi]
PST - ppublish
SO  - Osteoarthritis Cartilage. 2001 Feb;9(2):147-51. doi: 10.1053/joca.2000.0371.