PMID- 11238123
OWN - NLM
STAT- MEDLINE
DCOM- 20010426
LR  - 20210216
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 97
IP  - 6
DP  - 2001 Mar 15
TI  - UVB-induced apoptosis of human dendritic cells: contribution by caspase-dependent 
      and caspase-independent pathways.
PG  - 1803-8
AB  - Dendritic cells (DCs) play a central role in the initiation and regulation of the 
      immune response. The modalities by which DCs are committed to undergo apoptosis 
      are poorly defined. Here it is shown that, unlike death receptor ligands, UVB 
      radiation triggers apoptosis of human DCs very efficiently. UVB exposure is 
      followed by the activation of caspases 8, 9, and 3, by the loss of mitochondrial 
      transmembrane potential (deltaPsim), and by cellular and nuclear fragmentation. 
      Caspase inhibitors substantially prevented the occurrence of cellular and nuclear 
      fragmentation but had no effect on UVB-induced deltaPsim dissipation. 
      Importantly, mature DCs (MDCs) displayed relative resistance to UVB; UVB-induced 
      caspase activation and apoptosis were substantially delayed compared to immature 
      DCs (IDCs). Resistance correlated with the strong up-regulation of cellular FLIP 
      and bcl2 observed in MDCs compared to IDCs.
FAU - Nicolo, C
AU  - Nicolo C
AD  - Laboratory of Signal Transduction, Department of Experimental Medicine and 
      Biochemical Sciences, University of Rome Tor Vergata, Italy.
FAU - Tomassini, B
AU  - Tomassini B
FAU - Rippo, M R
AU  - Rippo MR
FAU - Testi, R
AU  - Testi R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (CASP8 and FADD-Like Apoptosis Regulating Protein)
RN  - 0 (CFLAR protein, human)
RN  - 0 (Carrier Proteins)
RN  - 0 (Intracellular Signaling Peptides and Proteins)
RN  - 0 (Proto-Oncogene Proteins c-bcl-2)
RN  - EC 3.4.22.- (Caspases)
SB  - IM
MH  - Apoptosis/*radiation effects
MH  - CASP8 and FADD-Like Apoptosis Regulating Protein
MH  - Carrier Proteins/pharmacology
MH  - Caspases/metabolism/pharmacology/physiology
MH  - Cell Culture Techniques
MH  - Cell Membrane Permeability/radiation effects
MH  - Dendritic Cells/enzymology/physiology/*radiation effects
MH  - Humans
MH  - Intracellular Membranes/radiation effects
MH  - *Intracellular Signaling Peptides and Proteins
MH  - Membrane Potentials/radiation effects
MH  - Mitochondria/radiation effects/ultrastructure
MH  - Proto-Oncogene Proteins c-bcl-2/pharmacology
MH  - *Ultraviolet Rays
EDAT- 2001/03/10 10:00
MHDA- 2001/05/01 10:01
CRDT- 2001/03/10 10:00
PHST- 2001/03/10 10:00 [pubmed]
PHST- 2001/05/01 10:01 [medline]
PHST- 2001/03/10 10:00 [entrez]
AID - S0006-4971(20)56200-9 [pii]
AID - 10.1182/blood.v97.6.1803 [doi]
PST - ppublish
SO  - Blood. 2001 Mar 15;97(6):1803-8. doi: 10.1182/blood.v97.6.1803.