PMID- 11239971
OWN - NLM
STAT- MEDLINE
DCOM- 20010412
LR  - 20190702
IS  - 0027-5107 (Print)
IS  - 0027-5107 (Linking)
VI  - 474
IP  - 1-2
DP  - 2001 Mar 1
TI  - In vitro transmission of chromosomal aberrations through mitosis in human 
      lymphocytes.
PG  - 139-46
AB  - Stable and unstable chromosome aberrations in human lymphocytes exposed to 2 and 
      4Gy of X-rays in G(0) were analyzed in M1 and M2 cells harvested at 72h to 
      investigate how the scoring protocol influences the yields of aberrations 
      transmitted through one mitosis. Metaphase chromosomes 2, 3, and 5 were painted 
      using fluorescence in situ hybridization (FISH) whole chromosome probes, together 
      with a pan-centromeric probe and stained by the harlequin-FISH method, to allow 
      the cell cycle status of each cell to be determined as it was scored. A strict 
      scoring criterion was adopted so that each metaphase had to contain 46 
      centromeres and each dicentric/centric ring had to have an acentric present. In 
      addition to scoring the painted material, unstable aberrations in the whole 
      genome were also recorded. The yield of complete dicentrics decreased by more 
      than a factor of 2 in going from M1 to M2. The decrease was greater at the lower 
      dose. Two-way translocations appeared stable, but one-way translocations 
      decreased. This suggests that if translocation yields are to be used for 
      biological dosimetry purposes, then the two-way type should be used.
FAU - Pala, F S
AU  - Pala FS
AD  - Cekmece Nuclear Research and Training Centre, P.K. 1 Ataturk Havaalani-34831, 
      Istanbul, Turkey. spala@atlas.net.tr
FAU - Moquet, J E
AU  - Moquet JE
FAU - Edwards, A A
AU  - Edwards AA
FAU - Lloyd, D C
AU  - Lloyd DC
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Mutat Res
JT  - Mutation research
JID - 0400763
SB  - IM
MH  - Adult
MH  - *Chromosome Aberrations
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Lymphocytes/radiation effects/*ultrastructure
MH  - Mitosis/*genetics
EDAT- 2001/03/10 10:00
MHDA- 2001/04/17 10:01
CRDT- 2001/03/10 10:00
PHST- 2001/03/10 10:00 [pubmed]
PHST- 2001/04/17 10:01 [medline]
PHST- 2001/03/10 10:00 [entrez]
AID - S002751070000172X [pii]
AID - 10.1016/s0027-5107(00)00172-x [doi]
PST - ppublish
SO  - Mutat Res. 2001 Mar 1;474(1-2):139-46. doi: 10.1016/s0027-5107(00)00172-x.