PMID- 11240039
OWN - NLM
STAT- MEDLINE
DCOM- 20010412
LR  - 20190826
IS  - 0165-5728 (Print)
IS  - 0165-5728 (Linking)
VI  - 114
IP  - 1-2
DP  - 2001 Mar 1
TI  - Interleukin-18 is induced in acute inflammatory demyelinating polyneuropathy.
PG  - 253-8
AB  - T lymphocytes of the Th1 subset producing the proinflammatory cytokine 
      interferon-gamma (IFN-gamma) have been implicated in the pathogenesis of 
      immune-mediated diseases of the peripheral nervous system (PNS) such as the acute 
      Guillain-Barre syndrome (GBS) and its animal model experimental autoimmune 
      neuritis (EAN). Interleukin-18 (IL-18) is a potent IFN-gamma-inducing cytokine 
      that is synthesized as an inactive precursor molecule and cleaved by caspase-1 
      into its mature active form. In our present study we analyzed the expression of 
      IL-18 and caspase-1 in the nerve roots of EAN rats using reverse 
      transcriptase-polymerase chain reaction and immunocytochemistry. Using an 
      enzyme-linked immunosorbent assay, we furthermore determined IL-18 protein levels 
      in paired serum and cerebrospinal fluid (CSF) samples from patients with GBS as 
      well as from noninflammatory neurologic disease (NIND) controls. In EAN, IL-18 
      and caspase-1 mRNA levels in the nerve roots increased during the stage of active 
      disease progression. Immunocytochemically, both perivascular and parenchymal 
      IL-18 protein expression was increased in the roots of EAN rats and mainly 
      associated with ED1+ macrophages stained on serial sections. IL-18 serum levels 
      were significantly higher in GBS patients than in NIND controls (238+/-71 vs. 
      42+/-7 pg/ml, P<0.001). Our data implicate the Th1-inducing cytokine IL-18 in the 
      pathogenesis of acute immune-mediated PNS demyelination.
FAU - Jander, S
AU  - Jander S
AD  - Department of Neurology, Heinrich-Heine-University, Moorenstrasse 5, D-40225 
      Dusseldorf, Germany. jander@uni-duesseldorf.de
FAU - Stoll, G
AU  - Stoll G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - J Neuroimmunol
JT  - Journal of neuroimmunology
JID - 8109498
RN  - 0 (Interleukin-18)
RN  - 0 (RNA, Messenger)
RN  - 82115-62-6 (Interferon-gamma)
RN  - EC 3.4.22.36 (Caspase 1)
SB  - IM
MH  - Animals
MH  - Caspase 1/genetics
MH  - Female
MH  - Gene Expression/immunology
MH  - Guillain-Barre Syndrome/blood/cerebrospinal fluid/*immunology
MH  - Immunohistochemistry
MH  - Interferon-gamma/immunology/metabolism
MH  - Interleukin-18/blood/cerebrospinal fluid/*genetics
MH  - RNA, Messenger/analysis
MH  - Rats
MH  - Rats, Inbred Lew
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Spinal Nerve Roots/immunology/metabolism
MH  - Th1 Cells/enzymology/*immunology
EDAT- 2001/03/10 10:00
MHDA- 2001/04/17 10:01
CRDT- 2001/03/10 10:00
PHST- 2001/03/10 10:00 [pubmed]
PHST- 2001/04/17 10:01 [medline]
PHST- 2001/03/10 10:00 [entrez]
AID - S0165572800004604 [pii]
AID - 10.1016/s0165-5728(00)00460-4 [doi]
PST - ppublish
SO  - J Neuroimmunol. 2001 Mar 1;114(1-2):253-8. doi: 10.1016/s0165-5728(00)00460-4.