PMID- 11241183 OWN - NLM STAT- MEDLINE DCOM- 20010419 LR - 20190709 IS - 0022-0795 (Print) IS - 0022-0795 (Linking) VI - 168 IP - 3 DP - 2001 Mar TI - NCX1 Na/Ca exchanger splice variants in pancreatic islet cells. PG - 517-26 AB - In the rat pancreatic beta-cell, Na/Ca exchange displays a quite high capacity. The cell is equipped with two alternatively spliced Na/Ca exchanger-1 (NCX1) isoforms, namely NCX1.3 and NCX1.7. To examine the existence of a possible functional difference between these splice variants, they were cloned, together with the heart variant NCX1.1, and expressed in human embryonic kidney-293 (HEK293) and Chinese hamster ovary (CHO) cells. In these two systems, the three splice variants showed a comparable level of intracellular Na+ (Na+(i))-dependent extracellular Ca2+(Ca2+(o)) uptake. Different levels of NCX1.3 and NCX1.7 transcripts were found in four rodent species, together with a marked interspecies difference in Na/Ca exchange activity. Three additional splice variants were found (NCX1.2, NCX1.9 and NCX1.13) in guinea-pigs, hamsters and mice, again in different proportions. Our data provide evidence for the activity of the three NCX1 splice variants. They also show the existence of a differential and species-specific transcription pattern of NCX1 gene products in pancreatic islet cells. FAU - Van Eylen, F AU - Van Eylen F AD - Laboratory of Pharmacology, Brussels University School of Medicine, Batiment GE, 808 route de Lennik, B-1070 Brussels, Belgium. FAU - Bollen, A AU - Bollen A FAU - Herchuelz, A AU - Herchuelz A LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - England TA - J Endocrinol JT - The Journal of endocrinology JID - 0375363 RN - 0 (Sodium-Calcium Exchanger) RN - 0 (sodium-calcium exchanger 1) RN - SY7Q814VUP (Calcium) SB - IM MH - *Alternative Splicing MH - Amino Acid Sequence MH - Animals MH - Base Sequence MH - Calcium/metabolism MH - Cell Culture Techniques MH - Cricetinae MH - Guinea Pigs MH - Humans MH - Islets of Langerhans/*metabolism MH - Mice MH - Molecular Sequence Data MH - Polymerase Chain Reaction MH - Rats MH - Sodium-Calcium Exchanger/chemistry/*genetics MH - Species Specificity MH - Transfection EDAT- 2001/03/10 10:00 MHDA- 2001/04/21 10:01 CRDT- 2001/03/10 10:00 PHST- 2001/03/10 10:00 [pubmed] PHST- 2001/04/21 10:01 [medline] PHST- 2001/03/10 10:00 [entrez] AID - JOE04024 [pii] AID - 10.1677/joe.0.1680517 [doi] PST - ppublish SO - J Endocrinol. 2001 Mar;168(3):517-26. doi: 10.1677/joe.0.1680517.