PMID- 11241233
OWN - NLM
STAT- MEDLINE
DCOM- 20010419
LR  - 20190620
IS  - 0008-543X (Print)
IS  - 0008-543X (Linking)
VI  - 91
IP  - 4
DP  - 2001 Feb 15
TI  - Genetic variation in the tumor necrosis factor-alpha promoter region and in the 
      stress protein hsp70-2: susceptibility and prognostic implications in breast 
      carcinoma.
PG  - 672-8
AB  - BACKGROUND: Tumor necrosis factor-alpha (TNF-alpha) and stress proteins (heat 
      shock proteins) are determining factors in the immune response to tumor cells. 
      The authors designated a large study to investigate the susceptibility and 
      prognostic implications of the genetic variation in TNF-alpha and hsp70-2 in 
      breast carcinoma. METHODS: The authors used the polymerase chain reaction and 
      restriction enzyme digestion to characterize the variation of the TNF-alpha 
      promoter region and that of the hsp70-2 gene in 243 unrelated Tunisian patients 
      with breast carcinoma and 174 healthy control subjects. Associations of the 
      clinicopathologic parameters and the genetic markers with the rates of the breast 
      carcinoma specific overall survival and the disease free survival (DFS) were 
      assessed using univariate and multivariate analyses. RESULTS: A highly 
      significant association was found between TNF2 homozygous genotype and breast 
      carcinoma (relative risk [RR], 4.44; P = 0.006). A high relative risk of breast 
      carcinoma was found to be associated with one hsp70-2 homozygous genotype (P2/P2; 
      RR, 7.12; P = 0.0001). The TNF2 homozygous genotype showed a significant 
      association with reduced DFS and/or overall survival by univariate test. 
      Conversely, P2-hsp70-2 homozygous genotype associated with increased overall 
      survival but not with DFS. Multivariate analysis retained significance for TNF2 
      homozygous genotype as an independent prognostic indicator for both DFS (RR, 
      2.75; P = 0.01) and overall survival (RR, 4.08; P = 0.01). CONCLUSIONS: Genetic 
      variation in TNF-alpha and hsp70-2 may represent not only markers for the 
      increased risk of breast carcinoma but also may predict the clinical outcome.
CI  - Copyright 2001 American Cancer Society.
FAU - Mestiri, S
AU  - Mestiri S
AD  - Laboratoire d'Immuno-Oncologie Moleculaire, Faculte de Medecine de Monastir, 
      Universite du Centre, 5019 Monastir, Tunisia.
FAU - Bouaouina, N
AU  - Bouaouina N
FAU - Ahmed, S B
AU  - Ahmed SB
FAU - Khedhaier, A
AU  - Khedhaier A
FAU - Jrad, B B
AU  - Jrad BB
FAU - Remadi, S
AU  - Remadi S
FAU - Chouchane, L
AU  - Chouchane L
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cancer
JT  - Cancer
JID - 0374236
RN  - 0 (HSP70 Heat-Shock Proteins)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Adult
MH  - Breast Neoplasms/*genetics/mortality/therapy
MH  - Breast Neoplasms, Male/genetics/mortality/therapy
MH  - Female
MH  - Genotype
MH  - HSP70 Heat-Shock Proteins/*genetics
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Multivariate Analysis
MH  - *Polymorphism, Genetic
MH  - Prognosis
MH  - Risk Factors
MH  - Survival Analysis
MH  - Tumor Necrosis Factor-alpha/*genetics
EDAT- 2001/03/10 10:00
MHDA- 2001/04/21 10:01
CRDT- 2001/03/10 10:00
PHST- 2001/03/10 10:00 [pubmed]
PHST- 2001/04/21 10:01 [medline]
PHST- 2001/03/10 10:00 [entrez]
AID - 10.1002/1097-0142(20010215)91:4<672::AID-CNCR1050>3.0.CO;2-J [pii]
AID - 10.1002/1097-0142(20010215)91:4<672::aid-cncr1050>3.0.co;2-j [doi]
PST - ppublish
SO  - Cancer. 2001 Feb 15;91(4):672-8. doi: 
      10.1002/1097-0142(20010215)91:4<672::aid-cncr1050>3.0.co;2-j.