PMID- 11241529
OWN - NLM
STAT- MEDLINE
DCOM- 20010621
LR  - 20190906
IS  - 0197-3851 (Print)
IS  - 0197-3851 (Linking)
VI  - 21
IP  - 2
DP  - 2001 Feb
TI  - Follow-up investigations in uncultured amniotic fluid cells after uncertain 
      cytogenetic results.
PG  - 75-80
AB  - Fluorescence in situ hybridization (FISH) on uncultured amniotic fluid (AF) cells 
      is a widespread technique for the rapid prenatal detection of specific chromosome 
      aberrations. During a 6-year period (1993-1998) we used FISH for quick follow-up 
      investigations in uncultured AF cells after finding an uncertain chromosome 
      aberration in a first chorionic villus (CV) or AF sample in 79 cases. These FISH 
      results were compared with conventional cytogenetic results of the AF cell 
      cultures in all cases. We found discrepant FISH and cytogenetic results in four 
      instances. In general, FISH on uncultured AF cells proved to be a reliable 
      technique for the rapid differentiation between confined placental mosaicism and 
      true fetal mosaicism, and between pseudomosaicism and true mosaicism, 
      respectively. Uncultured cells may sometimes even better reflect chromosomal 
      mosaicism than cultured cells, since they are not subject to culture induced 
      selection mechanisms. However, we found evidence that exceptional cases of tissue 
      confined mosaicism may go undetected in uncultured cells.
CI  - Copyright 2001 John Wiley & Sons, Ltd.
FAU - Van Opstal, D
AU  - Van Opstal D
AD  - Department of Clinical Genetics, University Hospital Dijkzigt, Erasmus 
      University, Rotterdam, The Netherlands. VANOPSTAL@KGEN.FGG.EUR.NL
FAU - van den Berg, C
AU  - van den Berg C
FAU - Galjaard, R J
AU  - Galjaard RJ
FAU - Los, F J
AU  - Los FJ
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - England
TA  - Prenat Diagn
JT  - Prenatal diagnosis
JID - 8106540
SB  - IM
MH  - Amniocentesis
MH  - Amniotic Fluid/*cytology
MH  - Chorionic Villi Sampling
MH  - *Chromosome Aberrations
MH  - Cytogenetic Analysis
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Mosaicism
MH  - Placenta/ultrastructure
MH  - Pregnancy
MH  - *Prenatal Diagnosis
MH  - Trisomy
EDAT- 2001/03/10 10:00
MHDA- 2001/06/22 10:01
CRDT- 2001/03/10 10:00
PHST- 2001/03/10 10:00 [pubmed]
PHST- 2001/06/22 10:01 [medline]
PHST- 2001/03/10 10:00 [entrez]
AID - 10.1002/1097-0223(200102)21:2<75::AID-PD990>3.0.CO;2-B [pii]
AID - 10.1002/1097-0223(200102)21:2<75::aid-pd990>3.3.co;2-2 [doi]
PST - ppublish
SO  - Prenat Diagn. 2001 Feb;21(2):75-80. doi: 
      10.1002/1097-0223(200102)21:2<75::aid-pd990>3.3.co;2-2.