PMID- 11241740
OWN - NLM
STAT- MEDLINE
DCOM- 20010517
LR  - 20220330
IS  - 0894-1491 (Print)
IS  - 0894-1491 (Linking)
VI  - 33
IP  - 3
DP  - 2001 Mar 1
TI  - Neurotrophic and migratory properties of an olfactory ensheathing cell line.
PG  - 225-9
AB  - Olfactory ensheathing cells (OECs) are a unique type of macroglia required for 
      normal olfactory axonal regeneration throughout the lifetime of an individual. 
      Recent evidence in the literature suggests that OECs transplanted into injured 
      spinal cords may facilitate axonal regeneration. In this study, we evaluated the 
      neurotrophic properties of OECs using a homogeneous clonal cell line (nOEC), 
      which does not contain contaminating cell types found in all primary OEC 
      cultures. The results indicate that nOECs express mRNA for NGF, BDNF, NT-4/5, and 
      neuregulins, but not for NT-3 or CNTF. In addition, nOECs secrete NGF, BDNF, and 
      neuregulin, but retain NT-4/5 intracellularly. Finally, prelabeled nOECs derived 
      from rat survived transplantation into a dorsal hemisected region of the hamster 
      spinal cord and migrated only in the injured, dorsal portion of the spinal cord. 
      This migratory pattern suggests that the nOECs are viable in vivo and respond to 
      signals originating from the injured neuronal cells and their processes.
CI  - Copyright 2001 Wiley-Liss, Inc.
FAU - Boruch, A V
AU  - Boruch AV
AD  - Department of Cell Biology, Neurobiology, and Anatomy, Stritch School of 
      Medicine, Loyola University Medical Center, 2160 South First Avenue, Maywood, IL 
      60153, USA.
FAU - Conners, J J
AU  - Conners JJ
FAU - Pipitone, M
AU  - Pipitone M
FAU - Deadwyler, G
AU  - Deadwyler G
FAU - Storer, P D
AU  - Storer PD
FAU - Devries, G H
AU  - Devries GH
FAU - Jones, K J
AU  - Jones KJ
LA  - eng
GR  - NS28238/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Glia
JT  - Glia
JID - 8806785
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Nerve Growth Factors)
RN  - 0 (Neuregulins)
RN  - 0 (RNA, Messenger)
RN  - 145172-44-7 (neurotrophin 5)
RN  - 9061-61-4 (Nerve Growth Factor)
RN  - P658DCA9XD (neurotrophin 4)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/genetics
MH  - Cell Line
MH  - Cell Movement/*physiology
MH  - Cricetinae
MH  - Gene Expression/physiology
MH  - Mesocricetus
MH  - Nerve Growth Factor/genetics
MH  - Nerve Growth Factors/*genetics
MH  - Nerve Regeneration/physiology
MH  - Neuregulins/genetics
MH  - Neuroglia/*cytology/physiology/*transplantation
MH  - Olfactory Mucosa/*cytology
MH  - RNA, Messenger/analysis
MH  - Rats
MH  - Spinal Cord Injuries/pathology
MH  - Transplantation, Heterologous
EDAT- 2001/03/10 10:00
MHDA- 2001/05/18 10:01
CRDT- 2001/03/10 10:00
PHST- 2001/03/10 10:00 [pubmed]
PHST- 2001/05/18 10:01 [medline]
PHST- 2001/03/10 10:00 [entrez]
AID - 10.1002/1098-1136(200103)33:3<225::AID-GLIA1021>3.0.CO;2-Y [pii]
PST - ppublish
SO  - Glia. 2001 Mar 1;33(3):225-9.