PMID- 11242648
OWN - NLM
STAT- MEDLINE
DCOM- 20010405
LR  - 20121115
IS  - 0385-0684 (Print)
IS  - 0385-0684 (Linking)
VI  - 28
IP  - 2
DP  - 2001 Feb
TI  - [Antitumor effect of the plant alkaloid preparation, cepharanthin].
PG  - 211-5
AB  - The antitumor effect of cepharanthin (CR), a biscoclaurine alkaloid, was examined 
      as to its direct action on tumor cells and inhibitory action on angiogenesis in 
      tumors. The effect of CR on in vitro invasion by murine RL-[symbol: see text] 1 
      leukemia cells and Colon 26 tumor cells was studied using a biocoat matrigel 
      invasion chamber. One hundred micrograms/ml of CR inhibited tumor cell invasion. 
      Early induction of apoptosis was assayed by the binding of annexin V and 
      phosphatidylserine (PS) in the cellular membrane CR (10 and 100 micrograms/ml) 
      induced apoptosis in human Daudi and Raji B lymphoblastoid cells. Treatment with 
      CR (1 and 10 micrograms/ml) also inhibited the in vitro growth of Daudi and Raji 
      cells. Ten micrograms/ml of CR also inhibited the growth of human umbilical vein 
      endothelial cells (HUVEC) and human dermal microvascular endothelial cells 
      (HMVEC). These results indicate that CR has diversified antitumor functions, 
      i.e., an enhancement of a sequential immune mechanism, a direct cytotoxic effect 
      and inhibitory action of angiogenesis in tumors.
FAU - Ebina, T
AU  - Ebina T
AD  - Division of Immunology, Research Institute Miyagi Cancer Center.
FAU - Ono, M
AU  - Ono M
LA  - jpn
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Japan
TA  - Gan To Kagaku Ryoho
JT  - Gan to kagaku ryoho. Cancer & chemotherapy
JID - 7810034
RN  - 0 (Alkaloids)
RN  - 0 (Angiogenesis Inhibitors)
RN  - 0 (Antineoplastic Agents, Phytogenic)
RN  - 0 (Benzylisoquinolines)
RN  - 7592YJ0J6T (cepharanthine)
SB  - IM
MH  - Alkaloids/*pharmacology
MH  - Angiogenesis Inhibitors/pharmacology
MH  - Antineoplastic Agents, Phytogenic/*pharmacology
MH  - Apoptosis/drug effects
MH  - Benzylisoquinolines
MH  - Drug Screening Assays, Antitumor
MH  - Humans
MH  - Neoplasm Invasiveness
MH  - Tumor Cells, Cultured
EDAT- 2001/03/13 10:00
MHDA- 2001/04/06 10:01
CRDT- 2001/03/13 10:00
PHST- 2001/03/13 10:00 [pubmed]
PHST- 2001/04/06 10:01 [medline]
PHST- 2001/03/13 10:00 [entrez]
PST - ppublish
SO  - Gan To Kagaku Ryoho. 2001 Feb;28(2):211-5.