PMID- 11243366
OWN - NLM
STAT- MEDLINE
DCOM- 20010524
LR  - 20190522
IS  - 1040-6387 (Print)
IS  - 1040-6387 (Linking)
VI  - 13
IP  - 1
DP  - 2001 Jan
TI  - Double in situ hybridization for simultaneous detection of porcine reproductive 
      and respiratory syndrome virus (PRRSV) and porcine circovirus (PCV).
PG  - 68-71
AB  - A double in situ hybridization method for the simultaneous detection of porcine 
      reproductive and respiratory syndrome virus (PRRSV) and porcine circovirus (PCV) 
      genomes in the same tissue section was applied to lung tissues from 9 pigs in 
      which PRRSV and PCV coinfection had been previously demonstrated. 
      Paraffin-embedded tissue sections were simultaneously hybridized with a 
      digoxigenin-labeled antisense RNA probe for PRRSV and a fluorescein-labeled 
      antisense RNA probe for PCV, and hybridization was detected with anti-digoxigenin 
      alkaline phosphatase/fast red and anti-fluorescein peroxidase/diaminobenzidine, 
      respectively. PRRSV and PCV genomes were identified in the same pulmonary cell 
      types as reported previously in all 9 pigs. In all pigs, PCV-positive cells 
      outnumbered PRRSV-positive cells. A small proportion of alveolar macrophages 
      contained both PRRSV and PCV genomes.
FAU - Sirinarumitr, T
AU  - Sirinarumitr T
AD  - Department of Veterinary Pathology, Faculty of Veterinary Medicine, Kasetsart 
      University, Kampangsaeng, Nakorn Pathom, Thailand.
FAU - Sorden, S D
AU  - Sorden SD
FAU - Morozov, I
AU  - Morozov I
FAU - Paul, P S
AU  - Paul PS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Vet Diagn Invest
JT  - Journal of veterinary diagnostic investigation : official publication of the 
      American Association of Veterinary Laboratory Diagnosticians, Inc
JID - 9011490
RN  - 0 (DNA, Viral)
RN  - NQ1SX9LNAU (Digoxigenin)
SB  - IM
MH  - Animals
MH  - Circoviridae Infections/diagnosis/genetics/veterinary
MH  - Circovirus/*genetics/isolation & purification
MH  - DNA, Viral/*analysis
MH  - Digoxigenin
MH  - In Situ Hybridization/methods/*veterinary
MH  - Lung/virology
MH  - Macrophages, Alveolar/virology
MH  - Porcine Reproductive and Respiratory Syndrome/diagnosis/genetics
MH  - Porcine respiratory and reproductive syndrome virus/*genetics/isolation & 
      purification
MH  - Sensitivity and Specificity
MH  - Swine
EDAT- 2001/03/13 10:00
MHDA- 2001/05/26 10:01
CRDT- 2001/03/13 10:00
PHST- 2001/03/13 10:00 [pubmed]
PHST- 2001/05/26 10:01 [medline]
PHST- 2001/03/13 10:00 [entrez]
AID - 10.1177/104063870101300113 [doi]
PST - ppublish
SO  - J Vet Diagn Invest. 2001 Jan;13(1):68-71. doi: 10.1177/104063870101300113.