PMID- 11244975
OWN - NLM
STAT- MEDLINE
DCOM- 20010419
LR  - 20160818
IS  - 0529-5807 (Print)
IS  - 0529-5807 (Linking)
VI  - 27
IP  - 3
DP  - 1998 Jun
TI  - [Effects of oxidatively modified lipoproteins on the expression of monocyte 
      chemoattractant protein-1 in endothelial cells].
PG  - 174-6
AB  - OBJECTIVE: To clarify whether lipoproteins, particularly oxidatively modified low 
      density lipoprotein (OX-LDL) and very low density lipoprotein (OX-VLDL) play a 
      role in the express of monocyte chemoattractant protein-1 (MCP-1) mRNA and 
      protein in endothelial cells (ECs). METHODS: After a 24-hour exposure to LDL, 
      OX-LDL, VLDL and OX-VLDL respectively the total RNA in call aorta ECs was 
      extracted by means of guanidinium isothiocyanate method. MCP-1 mRNA expression in 
      ECs was examined by dot blot analysis using a gamma-32P-end-labelled 35 mer 
      oligonucleotide probe of MCP-1. Meanwhile, MCP-1 protein in EC conditioned media 
      (EC-CM) of each group was determined by sandwich ELISA, and MCP-1 protein in ECs 
      was examined immunocytochemically as well. RESULTS: Cultured calf aorta ECs 
      expressed MCP-1 mRNA and protein, and OX-LDL and OX-VLDL induced further a strong 
      expression of MCP-1 mRNA, and an increased MCP-1 protein level in EC-CM. The 
      expression of MCP-1 mRNA and protein was only slightly increased when exposed to 
      LDL and VLDL, and immunohistological staining with polyclonal MCP-1 antibody give 
      a similar result. CONCLUSION: OX-LDL and OX-VLDL are able to induce a strong 
      expression of MCP-1 in ECs.
FAU - Yu, G
AU  - Yu G
AD  - Department of Pathology, Tongji Medical University, Wuhan 430030.
FAU - Deng, Z
AU  - Deng Z
FAU - Qu, Z
AU  - Qu Z
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhonghua Bing Li Xue Za Zhi
JT  - Zhonghua bing li xue za zhi = Chinese journal of pathology
JID - 0005331
RN  - 0 (Chemokine CCL2)
RN  - 0 (Lipoproteins, LDL)
RN  - 0 (Lipoproteins, VLDL)
RN  - 0 (RNA, Messenger)
RN  - 0 (oxidized low density lipoprotein)
RN  - 0 (oxidized very low density lipoprotein)
SB  - IM
MH  - Animals
MH  - Aorta, Thoracic/cytology/metabolism
MH  - Cattle
MH  - Cells, Cultured
MH  - Chemokine CCL2/*biosynthesis/genetics
MH  - Endothelium, Vascular/cytology/*metabolism
MH  - Lipoproteins, LDL/*pharmacology
MH  - Lipoproteins, VLDL/*pharmacology
MH  - RNA, Messenger/biosynthesis/genetics
EDAT- 2001/03/14 10:00
MHDA- 2001/04/21 10:01
CRDT- 2001/03/14 10:00
PHST- 2001/03/14 10:00 [pubmed]
PHST- 2001/04/21 10:01 [medline]
PHST- 2001/03/14 10:00 [entrez]
PST - ppublish
SO  - Zhonghua Bing Li Xue Za Zhi. 1998 Jun;27(3):174-6.