PMID- 11245223
OWN - NLM
STAT- MEDLINE
DCOM- 20010426
LR  - 20191104
IS  - 1341-7568 (Print)
IS  - 1341-7568 (Linking)
VI  - 75
IP  - 5
DP  - 2000 Oct
TI  - Chromosomal localization of 18S rDNA and telomere sequence in the aye-aye, 
      Daubentonia madagascariensis.
PG  - 299-303
AB  - Chromosomal localization of 18S rDNA and telomere sequence was attempted on the 
      chromosomes of the aye-aye (2n = 30) using fluorescence in situ hybridization 
      (FISH) and primed in situ labeling (PRINS), respectively. The rDNA was localized 
      at the tip or whole of the short arm of acrocentric chromosomes 13 and 14 in all 
      spreads observed. However, post-FISH silver-nitrate (Ag) staining showed that 
      transcriptional activity of the rRNA genes was variable, particularly in 
      chromosome 14, which was most frequently negative in one homologue carrying the 
      smaller copy number of rDNA. This observation supports, at the molecular 
      cytogenetic level, previous data concerning the relationship between the copy 
      number of rDNA and its trancriptional activity. On the other hand, telomere 
      sequence was localized only at the telomeric region of all chromosomes, the 
      so-called telomere-only pattern, a characteristic similar to that of the greater 
      bushbaby. These data may provide information on the chromosomal evolution of the 
      lemur, because locations of rDNA and telomere sequences frequently offer 
      important clues in reconstruction of karyotype differentiation.
FAU - Rakotoarisoa, G
AU  - Rakotoarisoa G
AD  - Primate Research Institute, Kyoto University, Inuyama, Aichi, Japan.
FAU - Hirai, Y
AU  - Hirai Y
FAU - Go, Y
AU  - Go Y
FAU - Kawamoto, Y
AU  - Kawamoto Y
FAU - Shima, T
AU  - Shima T
FAU - Koyama, N
AU  - Koyama N
FAU - Randrianjafy, A
AU  - Randrianjafy A
FAU - Mora, R
AU  - Mora R
FAU - Hirai, H
AU  - Hirai H
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Japan
TA  - Genes Genet Syst
JT  - Genes & genetic systems
JID - 9607822
RN  - 0 (DNA, Ribosomal)
RN  - 0 (RNA, Ribosomal, 18S)
SB  - IM
MH  - Animals
MH  - *Chromosome Mapping
MH  - Chromosomes/genetics
MH  - DNA, Ribosomal/*genetics
MH  - Evolution, Molecular
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Lemur/*genetics
MH  - Male
MH  - Nucleolus Organizer Region
MH  - RNA, Ribosomal, 18S/*genetics
MH  - Silver Staining
MH  - Telomere/*genetics
MH  - Transcription, Genetic
EDAT- 2001/03/14 10:00
MHDA- 2001/05/01 10:01
CRDT- 2001/03/14 10:00
PHST- 2001/03/14 10:00 [pubmed]
PHST- 2001/05/01 10:01 [medline]
PHST- 2001/03/14 10:00 [entrez]
AID - 10.1266/ggs.75.299 [doi]
PST - ppublish
SO  - Genes Genet Syst. 2000 Oct;75(5):299-303. doi: 10.1266/ggs.75.299.