PMID- 11246173
OWN - NLM
STAT- MEDLINE
DCOM- 20010405
LR  - 20191210
IS  - 0378-4274 (Print)
IS  - 0378-4274 (Linking)
VI  - 119
IP  - 3
DP  - 2001 Mar 8
TI  - Development of non-radio isotopic endpoint of murine local lymph node assay based 
      on 5-bromo-2'-deoxyuridine (BrdU) incorporation.
PG  - 203-8
AB  - Allergic contact dermatitis is a serious health problem. Over the last decade, 
      the murine local lymph node assay (LLNA) has been developed to detect chemical 
      allergens, and international validation studies have been conducted. We have 
      tried to establish an alternative non-radioisotopic endpoint for the LLNA by 
      using 5-bromo-2'-deoxyuridine (BrdU) incorporation in place of radioisotopes, 
      such as [3H]thymidine, employed in the standard method. BrdU was given as a 
      single administration at 5 mg/animal 2 days following three consecutive daily 
      applications of a test chemical. BrdU incorporation into draining lymph node 
      cells was measured using an enzyme immunosorbent assay technique. In this study, 
      p-benzoquinone(PBQ), trimellitic anhydride (TMA), citral(CT) and dextran (DEX) 
      were used as pilot chemicals. PBQ, TMA and CT, which are classified as moderate 
      to strong sensitizers in the guinea pig maximization test and were positive in 
      the original LLNA, were also found to elicit positive responses in the 
      alternative LLNA using BrdU incorporation. In contrast, DEX tested negative in 
      the modified assay consistent with previous guinea pig and LLNA data. 
      Consequently, the modified LLNA endpoint using BrdU incorporation may represent a 
      useful alternative to the standard assay in situations, where there is a need to 
      avoid the use of radioisotopes.
FAU - Takeyoshi, M
AU  - Takeyoshi M
AD  - Chemicals Assessment Center, Chemicals Evaluation and Research Institute, 3-822, 
      Ishii-machi, Oita 8770061, Hita-shi, Japan. takeyoshi-masahiro@hita.cerij.or.jp
FAU - Yamasaki, K
AU  - Yamasaki K
FAU - Yakabe, Y
AU  - Yakabe Y
FAU - Takatsuki, M
AU  - Takatsuki M
FAU - Kimber, I
AU  - Kimber I
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Toxicol Lett
JT  - Toxicology letters
JID - 7709027
RN  - 0 (Acyclic Monoterpenes)
RN  - 0 (Antimetabolites, Antineoplastic)
RN  - 0 (Benzoquinones)
RN  - 0 (Indicators and Reagents)
RN  - 0 (Monoterpenes)
RN  - 0 (Phthalic Anhydrides)
RN  - 0 (Terpenes)
RN  - 0 (Vasodilator Agents)
RN  - 3T006GV98U (quinone)
RN  - 80T61EUU7H (trimellitic anhydride)
RN  - G34N38R2N1 (Bromodeoxyuridine)
RN  - T7EU0O9VPP (citral)
SB  - IM
MH  - Acyclic Monoterpenes
MH  - Analysis of Variance
MH  - Animals
MH  - Antimetabolites, Antineoplastic/*metabolism
MH  - Benzoquinones
MH  - Bromodeoxyuridine/*metabolism
MH  - *Dermatitis, Allergic Contact
MH  - Endpoint Determination
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Indicators and Reagents/toxicity
MH  - *Local Lymph Node Assay
MH  - Lymph Nodes/*metabolism
MH  - Male
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - *Monoterpenes
MH  - Phthalic Anhydrides/toxicity
MH  - Sensitivity and Specificity
MH  - Terpenes/toxicity
MH  - Vasodilator Agents/toxicity
EDAT- 2001/03/14 10:00
MHDA- 2001/04/06 10:01
CRDT- 2001/03/14 10:00
PHST- 2001/03/14 10:00 [pubmed]
PHST- 2001/04/06 10:01 [medline]
PHST- 2001/03/14 10:00 [entrez]
AID - S0378427400003155 [pii]
AID - 10.1016/s0378-4274(00)00315-5 [doi]
PST - ppublish
SO  - Toxicol Lett. 2001 Mar 8;119(3):203-8. doi: 10.1016/s0378-4274(00)00315-5.