PMID- 11248328
OWN - NLM
STAT- MEDLINE
DCOM- 20010426
LR  - 20240104
IS  - 0145-2126 (Print)
IS  - 0145-2126 (Linking)
VI  - 25
IP  - 4
DP  - 2001 Apr
TI  - Complete karyotype characterization of the K562 cell line by combined application 
      of G-banding, multiplex-fluorescence in situ hybridization, fluorescence in situ 
      hybridization, and comparative genomic hybridization.
PG  - 313-22
AB  - This study combines conventional cytogenetics, fluorescence in situ hybridization 
      (FISH), multiplex-FISH and comparative genomic hybridization (CGH). In applying 
      this multimodal approach on the human leukemia cell line K562, the chromosome 
      composition was refined in detail and compared with data from the literature. A 
      hypotriploid karyotype with a modal chromosome number of 67, and 21 unique marker 
      chromosomes were identified. The classification of six markers was identical to 
      published data and the composition of five further markers from the literature 
      could be fully clarified for the first time. The composition of another five 
      markers, which have been interpreted in divergent ways in different studies, were 
      elucidated without doubt. Finally, five new markers of our study seem to have no 
      equivalents in former studies, very likely due to limitations of conventional 
      cytogenetics. The combinatory application of complementary techniques as shown in 
      this study will be very useful to provide the basis of a refined genotype 
      analysis on the chromosomal level.
FAU - Naumann, S
AU  - Naumann S
AD  - Department of Hematology, 3rd Medical Clinic, Johannes Gutenberg University of 
      Mainz, Verfuegungsgebaude, 911-SFB 519, Obere Zahlbacker Strasse 63, D55101, 
      Mainz, Germany.
FAU - Reutzel, D
AU  - Reutzel D
FAU - Speicher, M
AU  - Speicher M
FAU - Decker, H J
AU  - Decker HJ
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Leuk Res
JT  - Leukemia research
JID - 7706787
RN  - 0 (Genetic Markers)
SB  - IM
MH  - Chromosome Mapping/methods
MH  - Chromosome Painting/methods
MH  - Cytogenetics/*methods
MH  - Genetic Markers/genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/methods
MH  - K562 Cells/*metabolism/pathology/ultrastructure
MH  - Karyotyping/methods
MH  - Nucleic Acid Hybridization/methods
EDAT- 2001/03/15 10:00
MHDA- 2001/05/01 10:01
CRDT- 2001/03/15 10:00
PHST- 2001/03/15 10:00 [pubmed]
PHST- 2001/05/01 10:01 [medline]
PHST- 2001/03/15 10:00 [entrez]
AID - S0145-2126(00)00125-9 [pii]
AID - 10.1016/s0145-2126(00)00125-9 [doi]
PST - ppublish
SO  - Leuk Res. 2001 Apr;25(4):313-22. doi: 10.1016/s0145-2126(00)00125-9.