PMID- 11251315
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200930
IS  - 1386-6346 (Print)
IS  - 1386-6346 (Linking)
VI  - 19
IP  - 3
DP  - 2001 Mar 26
TI  - Competition of pravastatin and dibromosulfophthalein on the electroneutral and 
      electrogenic tetrabromosulfophthalein uptake in rat liver.
PG  - 336-346
AB  - The effect of pravastatin (PVST) and dibromosulfophthalein (DBSP) on the hepatic 
      uptake of bromosulfophthalein (BSP) was investigated in liver plasma membrane 
      vesicles enriched on basolateral fraction. BSP uptake was measured either in the 
      absence (electroneutral component) or in the presence of a membrane potential 
      (electrogenic component). PVST and DBSP inhibited both components of BSP uptake. 
      The Dixon and Cornish-Bowden plot of the data revealed a competitive type of 
      inhibition for PVST and DBSP on the electroneutral portion of BSP uptake. The 
      inhibition constants (K(i), mM) were 0.650+/-0.050 for PVST and 0.020+/-0.005 for 
      DBSP. These data indicate that PVST, DBSP and BSP share a common electroneutral 
      transport system at basolateral liver plasma membrane. On the contrary, PVST 
      exerted a non-competitive type of inhibition on the electrogenic component of BSP 
      uptake (K(i)=0.210+/-0.040 mM) and DBSP produced a mixed type of inhibition 
      (K(i)=0.018+/-0.002 mM). These results indicate that PVST does not compete with 
      the electrogenic BSP carrier system at the level of the substrate binding site 
      but modulates its functionality and that DBSP shares the electrogenic component 
      of BSP transport and also modulates the performance of this carrier system.
FAU - Torres, A M
AU  - Torres AM
AD  - Farmacologia, Facultad de Ciencias Bioquimicas y Farmaceuticas, Universidad 
      Nacional de Rosario, CONICET, Suipacha 531, 2000, Rosario, Argentina
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Hepatol Res
JT  - Hepatology research : the official journal of the Japan Society of Hepatology
JID - 9711801
EDAT- 2001/03/17 10:00
MHDA- 2001/03/17 10:01
CRDT- 2001/03/17 10:00
PHST- 2001/03/17 10:00 [pubmed]
PHST- 2001/03/17 10:01 [medline]
PHST- 2001/03/17 10:00 [entrez]
AID - S1386634600001030 [pii]
AID - 10.1016/s1386-6346(00)00103-0 [doi]
PST - ppublish
SO  - Hepatol Res. 2001 Mar 26;19(3):336-346. doi: 10.1016/s1386-6346(00)00103-0.