PMID- 11254712
OWN - NLM
STAT- MEDLINE
DCOM- 20010621
LR  - 20240325
IS  - 0022-1767 (Print)
IS  - 1550-6606 (Electronic)
IS  - 0022-1767 (Linking)
VI  - 166
IP  - 7
DP  - 2001 Apr 1
TI  - NF-kappa B p50-dependent in vivo footprints at Ig S gamma 3 DNA are correlated 
      with mu-->gamma 3 switch recombination.
PG  - 4552-9
AB  - NF-kappa B has been demonstrated to play critical roles in multiple aspects of 
      immune responses including Ig H chain isotype switching. To better define the 
      specific roles the p50 subunit of NF-kappa B plays in mu-->gamma 3 switch 
      recombination (SR), we systematically evaluated p50-deficient B cells for 
      activities that are strongly correlated with SR. B cell activation with LPS plus 
      anti-IgD-dextran plus IL-5 plus IL-4 plus TGF-beta produced normal levels of 
      proliferation and gamma3 germline transcripts in p50-deficient B cells, but 
      mu-->gamma 3 SR was impaired. In vitro binding studies previously showed that 
      NF-kappa B p50 homodimer binds the switch nuclear B-site protein (SNIP) of the S 
      gamma 3 tandem repeat. Ligation-mediated PCR in vivo footprint analysis 
      demonstrates that the region spanning the SNIP and switch nuclear A-site protein 
      (SNAP) binding sites of the S gamma 3 region are contacted by protein in normal 
      resting splenic B cells. B cells that are homozygous for the targeted disruption 
      of the gene encoding p50 (-/-) show strong aberrant footprints, whereas 
      heterozygous cells (+/-) reveal a partial effect in S gamma 3 DNA. These studies 
      provide evidence of nucleoprotein interactions at switch DNA in vivo and suggest 
      a direct interaction of p50 with S gamma 3 DNA that is strongly correlated with 
      SR competence.
FAU - Wuerffel, R A
AU  - Wuerffel RA
AD  - Department of Microbiology and Immunology, University of Illinois College of 
      Medicine, Chicago, IL 60680, USA.
FAU - Ma, L
AU  - Ma L
FAU - Kenter, A L
AU  - Kenter AL
LA  - eng
GR  - R01 AI045045/AI/NIAID NIH HHS/United States
GR  - R01 AI052400/AI/NIAID NIH HHS/United States
GR  - R01 AI052400-01A1/AI/NIAID NIH HHS/United States
GR  - GM 57078/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Immunoglobulin gamma-Chains)
RN  - 0 (Immunoglobulin mu-Chains)
RN  - 0 (NF-kappa B)
RN  - 0 (NF-kappa B p50 Subunit)
RN  - 0 (Nucleoproteins)
SB  - IM
MH  - Animals
MH  - B-Lymphocyte Subsets/cytology/immunology/metabolism
MH  - Base Sequence
MH  - Binding Sites/genetics/immunology
MH  - Cells, Cultured
MH  - *DNA Footprinting/methods
MH  - DNA-Binding Proteins/metabolism
MH  - Down-Regulation/genetics/immunology
MH  - Germ Cells/immunology
MH  - Immunoglobulin Class Switching/*genetics
MH  - Immunoglobulin Switch Region/*genetics
MH  - Immunoglobulin gamma-Chains/biosynthesis/*genetics/metabolism
MH  - Immunoglobulin mu-Chains/*genetics/metabolism
MH  - Interphase/immunology
MH  - Lymphocyte Activation/genetics
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Knockout
MH  - Mice, Nude
MH  - Molecular Sequence Data
MH  - NF-kappa B/deficiency/genetics/*physiology
MH  - NF-kappa B p50 Subunit
MH  - Nucleoproteins/metabolism
MH  - Recombination, Genetic/*immunology
MH  - Transcription, Genetic/immunology
PMC - PMC4975043
MID - NIHMS286823
EDAT- 2001/03/20 10:00
MHDA- 2001/06/22 10:01
PMCR- 2016/08/05
CRDT- 2001/03/20 10:00
PHST- 2001/03/20 10:00 [pubmed]
PHST- 2001/06/22 10:01 [medline]
PHST- 2001/03/20 10:00 [entrez]
PHST- 2016/08/05 00:00 [pmc-release]
AID - 10.4049/jimmunol.166.7.4552 [doi]
PST - ppublish
SO  - J Immunol. 2001 Apr 1;166(7):4552-9. doi: 10.4049/jimmunol.166.7.4552.