PMID- 11254946
OWN - NLM
STAT- MEDLINE
DCOM- 20010705
LR  - 20191104
IS  - 0166-445X (Print)
IS  - 0166-445X (Linking)
VI  - 53
IP  - 1
DP  - 2001 Jun
TI  - Experimental exposure of juvenile chinook (Oncorhynchus tshawytscha) to bleached 
      kraft mill effluent: hepatic CYP1A induction is correlated with DNA adducts but 
      not with organochlorine residues.
PG  - 49-63
AB  - Juvenile chinook salmon (Oncorhynchus tshawytscha) were exposed for 28 days to 
      0-16% treated effluent from an elemental chlorine free (ECF) bleached kraft pulp 
      mill. Fresh effluent was diluted with river water drawn from upstream of the 
      effluent diffuser. Fish were tested for biochemical responses to identify if the 
      effluent would cause significant effects at concentrations spanning those present 
      in the Fraser River, BC, Canada during winter conditions. Hepatic 
      ethoxyresorufin-O-deethylase (EROD) activity was increased significantly at all 
      effluent concentrations and hepatic cytochrome P450 1A (CYP1A) protein was 
      increased in all but 2% effluent. Hepatic DNA adduct concentrations were 
      increased significantly at 8 and 16% effluent. These data indicate that there is 
      a significant increase in all three responses at concentrations similar to those 
      found in the receiving waters (4%) and that a dose-response relationship exists 
      between BKME concentration and the responses measured. Carcasses contained low (< 
      0.2 pg g(-1)) concentrations of polychlorinated dibenzo-p-dioxins (PCDDs) and 
      polychlorinated dibenzofurans (PCDFs). Polychlorinated biphenyls (PCBs) were at 
      higher concentrations, accounting for 77% of the total contaminant burden, 
      expressed as TEQ, but contaminants do not appear to have bioaccumulated in this 
      study. It is likely that the PCB concentrations were due to contaminated 
      commercial fish feed. Bile did not contain detectable levels of polycyclic 
      aromatic hydrocarbons (PAHs) as measured by synchronous fluorescence 
      spectrometry.
FAU - Wilson, J Y
AU  - Wilson JY
AD  - Department of Fisheries and Oceans, Contaminants Science Section, Institute of 
      Ocean Sciences, PO Box 6000, BC, V8L 4B2, Sidney, Canada. jwilson@whoi.edu
FAU - Kruzynski, G M
AU  - Kruzynski GM
FAU - Addison, R F
AU  - Addison RF
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Aquat Toxicol
JT  - Aquatic toxicology (Amsterdam, Netherlands)
JID - 8500246
RN  - 0 (DNA Adducts)
RN  - EC 1.14.14.1 (Cytochrome P-450 CYP1A1)
RN  - EC 1.14.14.1 (Cytochrome P-450 CYP1A2)
SB  - IM
MH  - Animals
MH  - Cytochrome P-450 CYP1A1/*biosynthesis/metabolism
MH  - Cytochrome P-450 CYP1A2/*biosynthesis
MH  - DNA Adducts/*metabolism
MH  - Enzyme Induction/drug effects
MH  - *Extraction and Processing Industry
MH  - Liver/*enzymology
MH  - Oncorhynchus/*metabolism
MH  - Water Pollution, Chemical/*adverse effects
EDAT- 2001/03/20 10:00
MHDA- 2001/07/06 10:01
CRDT- 2001/03/20 10:00
PHST- 2001/03/20 10:00 [pubmed]
PHST- 2001/07/06 10:01 [medline]
PHST- 2001/03/20 10:00 [entrez]
AID - S0166-445X(00)00150-8 [pii]
AID - 10.1016/s0166-445x(00)00150-8 [doi]
PST - ppublish
SO  - Aquat Toxicol. 2001 Jun;53(1):49-63. doi: 10.1016/s0166-445x(00)00150-8.