PMID- 1125561
OWN - NLM
STAT- MEDLINE
DCOM- 19750811
LR  - 20190501
IS  - 0007-1447 (Print)
IS  - 0007-1447 (Linking)
VI  - 2
IP  - 5968
DP  - 1975 May 24
TI  - Which techniques should be used to screen blood donations for hepatitis B surface 
      antigen?
PG  - 412-4
AB  - Preservation of all donor sera already tested for Hepatitis B surface antigen 
      (HBsAg) by counterimmunoelectrophoresis (C.I.E.P.) allowed C.I.E.P., reversed 
      passive haemagglutination (R.P.H.A.), and radioimmunoassay (R.I.A.) to be 
      evaluated as screening techniques. Out of 165 811 donors tested for the first 
      time 207 were found by C.I.E.P. to be positive for HBsAf-a prevalence of 0-12%. 
      At the next donation 10 of those apparently negative on first screening were 
      HBsAg positive by C.I.E.P., and nine of these were shown by retesting with 
      R.P.H.A. and R.I.A. to have been positive at the earlier donation. These nine 
      false negatives caused four cases of transfusion-transmitted HBsAg-positive 
      hepatitis. On comparative screening of 22 239 donations C.I.E.P. detected 27 sera 
      positive for HBsAg, R.P.H.A. 39, and R.I.A. 41. Thus R.I.A. increased the 
      detection rate by more than half over C.I.E.P. From these 14 further 
      false-negative donations by C.I.E.P. six cases-one fatal-of HBsAg-positive 
      hepatitis occurred. The number of false positive reactions when using R.P.H.A. or 
      R.I.A. for screening was less than 1%. As many as 700 donations in one day have 
      been tested by R.P.H.A. or R.I.A. R.P.H.A. is faster and less expensive than 
      R.I.A., but R.I.A. is more objective. Either R.P.H.A. or R.I.A. should replace 
      C.I.E.P. as the routine method of screening donor sera for HBsAg.
FAU - Wallace, J
AU  - Wallace J
FAU - Barr, A
AU  - Barr A
FAU - Milne, G R
AU  - Milne GR
LA  - eng
PT  - Journal Article
PL  - England
TA  - Br Med J
JT  - British medical journal
JID - 0372673
RN  - 0 (Hepatitis B Antigens)
SB  - IM
MH  - *Blood Donors
MH  - False Negative Reactions
MH  - False Positive Reactions
MH  - Hemagglutination Tests/methods
MH  - Hepatitis B Antigens/*analysis
MH  - Humans
MH  - Immunoelectrophoresis/methods
MH  - Infant, Newborn
MH  - Radioimmunoassay/methods
MH  - Surface Properties
PMC - PMC1681838
EDAT- 1975/05/24 00:00
MHDA- 1975/05/24 00:01
PMCR- 1975/05/24
CRDT- 1975/05/24 00:00
PHST- 1975/05/24 00:00 [pubmed]
PHST- 1975/05/24 00:01 [medline]
PHST- 1975/05/24 00:00 [entrez]
PHST- 1975/05/24 00:00 [pmc-release]
AID - 10.1136/bmj.2.5968.412 [doi]
PST - ppublish
SO  - Br Med J. 1975 May 24;2(5968):412-4. doi: 10.1136/bmj.2.5968.412.