PMID- 11256472
OWN - NLM
STAT- MEDLINE
DCOM- 20010628
LR  - 20190719
IS  - 0918-6158 (Print)
IS  - 0918-6158 (Linking)
VI  - 24
IP  - 3
DP  - 2001 Mar
TI  - Quantitative measurement of the novel human plasma protein, IHRP, by sandwich 
      ELISA.
PG  - 214-7
AB  - Inter-alpha-trypsin inhibitor family heavy chain-related protein (IHRP), which 
      has a sequence similarity to the heavy chains of the inter-alpha-trypsin 
      inhibitor (ITI) family, is a novel glycoprotein found in human plasma. We 
      prepared two clones (1A4 and 6E11) of anti-IHRP mouse monoclonal antibody. Both 
      of them recognized the C-terminal 35-kDa fragment which was produced by plasma 
      kallikrein-digestion of IHRP. We developed a sandwich ELISA for measurement of 
      the plasma IHRP concentration with the monoclonal antibody coated microtiter 
      plate and the anti-N-terminal 57-kDa fragment of IHRP rabbit polyclonal antibody 
      (anti-GP57). We found that the average concentration of IHRP in the plasma of 
      healthy donors was 101.3+/-31.8 microg/ml (average+/-S.D.). The IHRP 
      concentration in the plasma of patients with inflammatory disorders was slightly 
      increased (137.5+/-40.2 microg/ml: average+/-S.D.). Together with the previous 
      data indicating the induction of the porcine mRNA, which is thought to be the 
      species counterpart of human IHRP mRNA, in the liver after resuscitation from 
      cardiogenic shock, we propose that IHRP is a member of the acute-phase protein 
      family.
FAU - Choi-Miura, N H
AU  - Choi-Miura NH
AD  - Department of Physiological Chemistry, School of Pharmaceutical Sciences, Showa 
      University, Shinagawa, Tokyo, Japan. nammiura@pharm.showa-u.ac.jp
LA  - eng
PT  - Journal Article
PL  - Japan
TA  - Biol Pharm Bull
JT  - Biological & pharmaceutical bulletin
JID - 9311984
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Blood Proteins)
RN  - 0 (Glycoproteins)
RN  - 0 (ITIH4 protein, human)
RN  - 0 (Proteinase Inhibitory Proteins, Secretory)
SB  - IM
MH  - Animals
MH  - Antibodies, Monoclonal/chemistry
MH  - Blood Proteins/*chemistry
MH  - Blotting, Western
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Glycoproteins/*chemistry
MH  - Inflammation/metabolism
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Proteinase Inhibitory Proteins, Secretory
EDAT- 2001/03/21 10:00
MHDA- 2001/06/29 10:01
CRDT- 2001/03/21 10:00
PHST- 2001/03/21 10:00 [pubmed]
PHST- 2001/06/29 10:01 [medline]
PHST- 2001/03/21 10:00 [entrez]
AID - 10.1248/bpb.24.214 [doi]
PST - ppublish
SO  - Biol Pharm Bull. 2001 Mar;24(3):214-7. doi: 10.1248/bpb.24.214.