PMID- 11257209
OWN - NLM
STAT- MEDLINE
DCOM- 20010621
LR  - 20211203
IS  - 1084-8592 (Print)
IS  - 1084-8592 (Linking)
VI  - 6
IP  - 1
DP  - 2001 Mar
TI  - Comparative genomic hybridization of hepatocellular carcinoma: correlation with 
      fluorescence in situ hybridization in paraffin-embedded tissue.
PG  - 27-37
AB  - BACKGROUND: Proto-oncogene MYC, mapped to chromosomal band 8q24 and the genes for 
      hepatocyte growth factor (HGF at 7q21) and its receptor, MET, at chromosomal band 
      7q31, have an important role in the biology and growth of normal and neoplastic 
      liver. Comparative genomic hybridization (CGH) and fluorescence in situ 
      hybridization (FISH) studies have reported frequent abnormalities of chromosomes 
      1 and 8 in hepatocellular carcinomas (HCCs) of various clinical and pathological 
      stages. Chromosome 7 involvement is reported to be less frequent. MATERIALS AND 
      METHODS: Frozen tissue from 17 HCCs was used for CGH analysis and sections of 
      corresponding formalin-fixed, paraffin-embedded HCC tissue were used for 
      dual-color FISH with locus-specific (LSI-cMYC for chromosome 8q24 and LSI D7S486 
      for chromosome 7q31) and centromeric probes, CEP8 (8p11.1-q11.2) and CEP7 
      (7p11.1-q11.2) (Vysis, Inc, Downers Grove, IL). This study intended to determine 
      the pattern of chromosomal aberrations in early-stage (incidental) HCC and large 
      surgically resected HCC, and also compared the efficiency and usefulness of the 
      two cytogenetic methods. RESULTS: CGH showed abnormalities on chromosomes 1q, 5q, 
      7q, 8q, 9, 10, 13q, 15, 16, 17p, 18q, 19, 20, 21, 22, and X. Gains of 8q were 
      noted in 50% of the HCCs, including five cases of incidental HCCs by CGH. 
      Increase in copy numbers of MYC detected by FISH was noted in 25% of tumors that 
      had shown 8q gains by CGH and in five cases with no chromosome abnormalities 
      noted by CGH. Three cases with 7q31 copy number abnormalities were found by FISH 
      in addition to those detected by CGH. CONCLUSION: Combined use of CGH and FISH 
      may provide important information about early and/or primary genetic changes in 
      the development of HCC.
FAU - Rao, U N
AU  - Rao UN
AD  - Department of Pathology, Presbyterian University Hospital, University of 
      Pittsburgh Medical Center, PA 15213, USA. raounm@MSX.UPMC.EDU
FAU - Gollin, S M
AU  - Gollin SM
FAU - Beaves, S
AU  - Beaves S
FAU - Cieply, K
AU  - Cieply K
FAU - Nalesnik, M
AU  - Nalesnik M
FAU - Michalopoulos, G K
AU  - Michalopoulos GK
LA  - eng
GR  - P30 CA47904/CA/NCI NIH HHS/United States
GR  - S10RR11879/RR/NCRR NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Mol Diagn
JT  - Molecular diagnosis : a journal devoted to the understanding of human disease 
      through the clinical application of molecular biology
JID - 9614965
SB  - IM
MH  - Adolescent
MH  - Adrenal Gland Neoplasms/genetics/secondary
MH  - Adult
MH  - Aged
MH  - Carcinoma, Hepatocellular/*genetics/pathology
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Liver Neoplasms/*genetics/pathology
MH  - Lung Neoplasms/genetics/secondary
MH  - Male
MH  - Middle Aged
MH  - Nucleic Acid Hybridization/*methods
MH  - Paraffin Embedding
MH  - Proto-Oncogene Mas
EDAT- 2001/03/21 10:00
MHDA- 2001/06/22 10:01
CRDT- 2001/03/21 10:00
PHST- 2001/03/21 10:00 [pubmed]
PHST- 2001/06/22 10:01 [medline]
PHST- 2001/03/21 10:00 [entrez]
AID - S1084-8592(01)30739-7 [pii]
AID - 10.1054/modi.2001.22021 [doi]
PST - ppublish
SO  - Mol Diagn. 2001 Mar;6(1):27-37. doi: 10.1054/modi.2001.22021.