PMID- 11257612
OWN - NLM
STAT- MEDLINE
DCOM- 20010531
LR  - 20191104
IS  - 0022-3034 (Print)
IS  - 0022-3034 (Linking)
VI  - 47
IP  - 1
DP  - 2001 Apr
TI  - Phosphatidylinositol-3-OH kinase regulatory subunits are differentially expressed 
      during development of the rat cerebellum.
PG  - 39-50
AB  - Recent evidence implicates a central role for PI3K signalling in mediating cell 
      survival during the process of neuronal differentiation. Although PI3K activity 
      is stimulated by a wide range of growth factors and cytokines in different cell 
      lines and tissues, activation of this pathway by insulin-like growth factor I 
      (IGF-I) most likely represents the main survival signal during neuronal 
      differentiation. IGF-I is highly expressed during development of the central 
      nervous system, and thus is a critical factor for the development and maturation 
      of the cerebellum. Upon ligand binding, the IGF-I receptor phosphorylates 
      tyrosine residues in SHC and insulin receptor substrates (IRSs) initiating two 
      main signalling cascades, the MAP kinase and the phosphatidylinositol 3-kinase 
      (PI3K) pathways. Activated PI3K is composed of a catalytic subunit (p110alpha or 
      beta) associated with one of a large family of regulatory subunits (p85alpha, 
      p85beta, p55gamma, p55alpha, and p50alpha). To evaluate the contributions of 
      these various regulatory subunits to neuronal differentiation, we have used 
      antibodies specific for each of the PI3K subunits. Using these antisera, we now 
      demonstrate that PI3K subunits are differentially regulated in cerebellar 
      development, and that the expression level of the p55gamma regulatory subunit 
      reaches a maximum during postnatal development, decreasing thereafter to low 
      levels in the adult cerebellum. Furthermore, our studies reveal that the 
      distribution of the various PI3K regulatory subunits varies during development of 
      the cerebellum. Interestingly, p55gamma is expressed in both glial and neuronal 
      cells; moreover, in Purkinje neurones, this subunit colocalises with the IGF-IR.
CI  - Copyright 2001 John Wiley & Sons, Inc.
FAU - Trejo, J L
AU  - Trejo JL
AD  - Cellular and Molecular Neuroendocrinology Laboratory, Instituto Cajal de 
      Neurobiologia, C.S.I.C., Av. Doctor Arce 37, Madrid E28002, Spain.
FAU - Pons, S
AU  - Pons S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Neurobiol
JT  - Journal of neurobiology
JID - 0213640
RN  - 0 (Protein Subunits)
RN  - EC 2.7.1.- (Phosphatidylinositol 3-Kinases)
RN  - EC 2.7.10.1 (Receptor, IGF Type 1)
SB  - IM
MH  - Animals
MH  - Antibody Specificity
MH  - Blotting, Western
MH  - COS Cells
MH  - Cerebellum/*enzymology/*growth & development
MH  - Gene Expression Regulation, Developmental
MH  - Immunohistochemistry
MH  - Neuroglia/cytology/metabolism
MH  - Neurons/cytology/metabolism
MH  - Phosphatidylinositol 3-Kinases/*biosynthesis/genetics
MH  - *Protein Subunits
MH  - Purkinje Cells/cytology/metabolism
MH  - Rats
MH  - Rats, Wistar
MH  - Receptor, IGF Type 1/metabolism
EDAT- 2001/03/21 10:00
MHDA- 2001/06/02 10:01
CRDT- 2001/03/21 10:00
PHST- 2001/03/21 10:00 [pubmed]
PHST- 2001/06/02 10:01 [medline]
PHST- 2001/03/21 10:00 [entrez]
AID - 10.1002/neu.1014 [pii]
AID - 10.1002/neu.1014 [doi]
PST - ppublish
SO  - J Neurobiol. 2001 Apr;47(1):39-50. doi: 10.1002/neu.1014.