PMID- 11260321
OWN - NLM
STAT- MEDLINE
DCOM- 20010405
LR  - 20190513
IS  - 0019-2805 (Print)
IS  - 1365-2567 (Electronic)
IS  - 0019-2805 (Linking)
VI  - 102
IP  - 2
DP  - 2001 Feb
TI  - Capacity of mouse mast cells to prime T cells and to induce specific antibody 
      responses in vivo.
PG  - 165-72
AB  - Mouse, human and rat mast cells have been shown to express major 
      histocompatibility complex II molecules and present antigens to specific T-cell 
      hybridomas in vitro. The purpose of our investigation was to determine whether 
      mouse mast cells are able to initiate specific immune responses in vivo. 
      Induction of anti-dinitrophenyl (DNP) immunoglobulin G1 (IgG1) and IgG2a 
      antibodies was performed by transferring ovalbumin (OVA)-DNP-pulsed bone 
      marrow-derived mast cells (BMMC), B cells, or macrophages into naive mice which 
      were boosted later with soluble antigen. Cultured spleen cells from immunized 
      mice were tested for their cytokine content. Our data show that mast cells were 
      by far better inducers of anti-DNP IgG1 antibodies than were B cells and 
      macrophages. In contrast, anti-DNP IgG2a response induced by macrophages was much 
      stronger than that obtained with mast cells whereas B cells were completely 
      unable to elicit this response. In addition to a high index of cell 
      proliferation, spleen cells from mast cell-injected mice produced more 
      interferon-gamma than those mice who received macrophages or B cells by two- to 
      fivefold, and almost 10-fold, respectively. Mast cell-deficient Wf/Wf mice were 
      compared with their normal +/+ littermates and with mast cell-reconstituted Wf/Wf 
      mice to develop delayed-type hypersensitivity (DTH) reactions as well as humoral 
      immune responses. Mast cell sufficient mice as well as mast cell-reconstituted 
      Wf/Wf mice developed significantly increased DTH reactions (P = 0.02, and 0.03, 
      respectively) and higher anti-OVA-specific antibody responses as compared with 
      Wf/Wf mice. Our data suggest that mast cells have the potential to up-regulate 
      both humoral and cellular immune responses in vivo.
FAU - Villa, I
AU  - Villa I
AD  - Unites d'Immuno-allergy, Histopathology, Institut Pasteur, Paris, France.
FAU - Skokos, D
AU  - Skokos D
FAU - Tkaczyk, C
AU  - Tkaczyk C
FAU - Peronet, R
AU  - Peronet R
FAU - David, B
AU  - David B
FAU - Huerre, M
AU  - Huerre M
FAU - Mecheri, S
AU  - Mecheri S
LA  - eng
PT  - Journal Article
PL  - England
TA  - Immunology
JT  - Immunology
JID - 0374672
RN  - 0 (Dinitrophenols)
RN  - 0 (Haptens)
RN  - 0 (Immunoglobulin G)
RN  - 0 (dinitrophenol-ovalbumin)
RN  - 82115-62-6 (Interferon-gamma)
RN  - 9006-59-1 (Ovalbumin)
SB  - IM
MH  - Animals
MH  - Antigen-Presenting Cells/*immunology
MH  - B-Lymphocytes/immunology
MH  - Cell Culture Techniques
MH  - Cell Division/immunology
MH  - Dinitrophenols/immunology
MH  - Dose-Response Relationship, Immunologic
MH  - Female
MH  - Haptens/immunology
MH  - Hypersensitivity, Delayed/immunology
MH  - Immunization
MH  - Immunoglobulin G/*biosynthesis
MH  - Interferon-gamma/biosynthesis
MH  - Lymphocyte Activation/immunology
MH  - Macrophages/immunology
MH  - Mast Cells/*immunology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Ovalbumin/immunology
MH  - T-Lymphocytes/*immunology
PMC - PMC1783168
EDAT- 2001/03/22 10:00
MHDA- 2001/04/06 10:01
PMCR- 2002/02/01
CRDT- 2001/03/22 10:00
PHST- 2001/03/22 10:00 [pubmed]
PHST- 2001/04/06 10:01 [medline]
PHST- 2001/03/22 10:00 [entrez]
PHST- 2002/02/01 00:00 [pmc-release]
AID - imm178 [pii]
AID - 10.1046/j.1365-2567.2001.01178.x [doi]
PST - ppublish
SO  - Immunology. 2001 Feb;102(2):165-72. doi: 10.1046/j.1365-2567.2001.01178.x.