PMID- 11260327
OWN - NLM
STAT- MEDLINE
DCOM- 20010405
LR  - 20240413
IS  - 0019-2805 (Print)
IS  - 1365-2567 (Electronic)
IS  - 0019-2805 (Linking)
VI  - 102
IP  - 2
DP  - 2001 Feb
TI  - Impaired protective immunity and T helper 2 responses in alymphoplasia (aly) 
      mutant mice infected with Trichinella spiralis.
PG  - 218-24
AB  - The alymphoplasia (aly) mutation of mice prevents the development of systemic 
      lymph nodes and Peyer's patches. The mutant homozygotes (aly/aly) are partially 
      deficient in both humoral and cell-mediated immune functions. In the present 
      study, we show that adult worm expulsion was slightly delayed and that T helper 2 
      (Th2)-type responses were partially defective in aly/aly mice after infection 
      with Trichinella spiralis. Male aly/aly and aly/+ mice (8-weeks old) were 
      infected with 400 muscle larvae. There was no difference in worm recovery between 
      the two groups on day 5. However, worm recovery in aly/aly mice was significantly 
      higher than that in aly/+ mice on day 14. Mucosal mast cells increased in number 
      and peaked 14 days after infection in aly/+ mice. aly/aly mice were deficient in 
      their mucosal mast cell response through out the primary infection. To examine 
      the existence of mast cell precursors, aly/aly mice were treated with recombinant 
      interleukin-3 (rIL-3) before infection. The mast cell response was poorly induced 
      in aly/aly mice treated with rIL-3. An immunoglobulin E (IgE) response was not 
      detected in aly/aly mice during the course of infection. Serum IgG1 levels in 
      aly/aly mice were significantly lower than that of aly/+. The serum IgG2a levels 
      increased in both strains of mice. However, IgG2a production in aly/aly mice on 
      day 14 was half as much as that in aly/+mice. Stimulation of splenic T cells in 
      vitro with anti-CD3 monoclonal antibody (mAb) showed that spleen cells from aly/+ 
      mice on day 5 produced more IL-4 than spleen cells from aly/aly mice. IL-4 
      production from aly/aly mice on day 14 was half that from aly/+ mice. 
      Interferon-gamma (IFN-gamma) was produced in both aly/aly and aly/+ mice on day 
      14. Proliferation assay showed that T cells of aly/aly mice responded poorly when 
      cultured with antigen-presenting cells. These results suggest that aly gene is 
      needed for the induction of protective immunity and Th2 responses in mice 
      infected with T. spiralis.
FAU - Korenaga, M
AU  - Korenaga M
AD  - Department of Parasitology, Kochi Medical School, Nankoku City, Kochi, Japan.
FAU - Akimaru, Y
AU  - Akimaru Y
FAU - Shamsuzzaman, S M
AU  - Shamsuzzaman SM
FAU - Hashiguchi, Y
AU  - Hashiguchi Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Immunology
JT  - Immunology
JID - 0374672
RN  - 0 (Immunoglobulin G)
RN  - 207137-56-2 (Interleukin-4)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Animals
MH  - Cell Culture Techniques
MH  - Cell Division/immunology
MH  - Immunity, Mucosal
MH  - Immunoglobulin E/blood
MH  - Immunoglobulin G/blood
MH  - Immunologic Deficiency Syndromes/*immunology
MH  - Interferon-gamma/biosynthesis
MH  - Interleukin-4/biosynthesis
MH  - Intestinal Mucosa/immunology
MH  - Male
MH  - Mast Cells/immunology
MH  - Mice
MH  - Mice, Mutant Strains
MH  - Point Mutation
MH  - Spleen/immunology
MH  - Th2 Cells/*immunology
MH  - *Trichinella spiralis/isolation & purification
MH  - Trichinellosis/*immunology
PMC - PMC1783161
EDAT- 2001/03/22 10:00
MHDA- 2001/04/06 10:01
PMCR- 2002/02/01
CRDT- 2001/03/22 10:00
PHST- 2001/03/22 10:00 [pubmed]
PHST- 2001/04/06 10:01 [medline]
PHST- 2001/03/22 10:00 [entrez]
PHST- 2002/02/01 00:00 [pmc-release]
AID - imm169 [pii]
AID - 10.1046/j.1365-2567.2001.01169.x [doi]
PST - ppublish
SO  - Immunology. 2001 Feb;102(2):218-24. doi: 10.1046/j.1365-2567.2001.01169.x.