PMID- 11260329
OWN - NLM
STAT- MEDLINE
DCOM- 20010405
LR  - 20240413
IS  - 0019-2805 (Print)
IS  - 1365-2567 (Electronic)
IS  - 0019-2805 (Linking)
VI  - 102
IP  - 2
DP  - 2001 Feb
TI  - Induction of in vivo resistance to Mycobacterium avium infection by intramuscular 
      injection with DNA encoding interleukin-18.
PG  - 234-41
AB  - Interferon-gamma (IFN-gamma) is closely associated with the generation of 
      cell-mediated immunity and resistance to intracellular parasites. Interleukin-18 
      (IL-18) was known to strongly induce IFN-gamma production by T cells and natural 
      killer (NK) cells. In order to determine whether injection with DNA encoding 
      IL-18 can stimulate the resistance to Mycobacterium avium complex (MAC) 
      infection, the mature IL-18 cDNA with kappa leader sequence was cloned under 
      control of the cytomegalovirus (CMV) promoter (TcCMVIL-18) and its effect on MAC 
      infection was investigated in genetically susceptible BALB/c mice. Injection with 
      the TcCMVIL-18 DNA during intranasal infection with MAC resulted in a significant 
      decrease in bacterial load of lung during the entire 8-week observation period, 
      while injection with the TcCMV control DNA did not. Lung cells in mice injected 
      with the TcCMVIL-18 DNA showed persistent production of IFN-gamma throughout the 
      8-week period. Furthermore, immunization with the TcCMVIL-18 DNA induced and 
      maintained significantly higher levels of cytotoxic activity and nitric oxide 
      production by lung cells than immunization with the TcCMV control vector. This 
      work suggests that IL-18 DNA vaccination may be useful in the immunotherapeutic 
      or immunoprotection approaches of infections by intracellular parasites such as 
      mycobacteria.
FAU - Kim, S H
AU  - Kim SH
AD  - College of Pharmacy, Chonnam National University, Kwangju, and Cancer Research 
      Center, Seoul National University College of Medicine, Seoul, Republic of Korea.
FAU - Cho, D
AU  - Cho D
FAU - Kim, T S
AU  - Kim TS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Immunology
JT  - Immunology
JID - 0374672
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Bacterial Vaccines)
RN  - 0 (DNA, Complementary)
RN  - 0 (Interleukin-18)
RN  - 0 (Vaccines, DNA)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Animals
MH  - Antibodies, Monoclonal/immunology
MH  - Bacterial Vaccines/*immunology
MH  - DNA, Complementary/genetics
MH  - Female
MH  - Injections, Intramuscular
MH  - Interferon-gamma/biosynthesis/immunology
MH  - Interleukin-18/genetics/*immunology
MH  - Lung/immunology/microbiology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mycobacterium Infections/immunology/*prevention & control
MH  - Mycobacterium avium/growth & development/*immunology
MH  - Transfection
MH  - Vaccines, DNA/*immunology
PMC - PMC1783174
EDAT- 2001/03/22 10:00
MHDA- 2001/04/06 10:01
PMCR- 2002/02/01
CRDT- 2001/03/22 10:00
PHST- 2001/03/22 10:00 [pubmed]
PHST- 2001/04/06 10:01 [medline]
PHST- 2001/03/22 10:00 [entrez]
PHST- 2002/02/01 00:00 [pmc-release]
AID - imm150 [pii]
AID - 10.1046/j.1365-2567.2001.01150.x [doi]
PST - ppublish
SO  - Immunology. 2001 Feb;102(2):234-41. doi: 10.1046/j.1365-2567.2001.01150.x.