PMID- 11260601
OWN - NLM
STAT- MEDLINE
DCOM- 20010719
LR  - 20191210
IS  - 0197-3851 (Print)
IS  - 0197-3851 (Linking)
VI  - 21
IP  - 3
DP  - 2001 Mar
TI  - Prenatal diagnosis on fetal cells from maternal blood: practical comparative 
      evaluation of the first and second trimesters.
PG  - 165-70
AB  - Objectives- Several attempts have been made to determine the gestational period 
      in which the maximum number of fetal cells can be found in maternal blood and 
      consequently which is the best week in which to perform a reliable non-invasive 
      prenatal diagnosis. Most studies conclude that the number of nucleated red blood 
      cells (NRBC) increases in line with gestation, but the number of cells that are 
      fetal in origin (FNRBC) decreases in the third trimester. The aim of the present 
      study was to make a practical comparative evaluation of the first and second 
      trimesters to ascertain the period in which a greater number of FNRBC can be 
      found of the total number of NRBC identified. Methods- Double density gradient 
      and a posterior positive selection (CD71) by magnetic activated cell sorting 
      (MACS) were employed. In the final fraction, erythroblasts were identified using 
      Kleihauer staining and were studied using the fluorescence in situ hybridization 
      (FISH) interphasic technique. Results- There was a significant difference 
      (p<0.05) between the mean number of FNRBC found in the first and second 
      trimesters. Conclusions- The number of FNRBC increases from the first to the 
      second trimester. It appears that the optimum week in which to perform a reliable 
      non-invasive prenatal diagnosis is around the 15th week.
CI  - Copyright 2001 John Wiley & Sons, Ltd.
FAU - Rodriguez de Alba, M
AU  - Rodriguez de Alba M
AD  - Department of Genetics, Fundacion Jimenez Diaz, Avda. Reyes Catolicos no. 2, 
      Madrid 28040, Spain. cramosa@fjd.es
FAU - Palomino, P
AU  - Palomino P
FAU - Gonzalez-Gonzalez, C
AU  - Gonzalez-Gonzalez C
FAU - Lorda-Sanchez, I
AU  - Lorda-Sanchez I
FAU - Ibanez, M A
AU  - Ibanez MA
FAU - Sanz, R
AU  - Sanz R
FAU - Fernandez-Moya, J M
AU  - Fernandez-Moya JM
FAU - Ayuso, C
AU  - Ayuso C
FAU - Diaz-Recasens, J
AU  - Diaz-Recasens J
FAU - Ramos, C
AU  - Ramos C
LA  - eng
PT  - Comparative Study
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Prenat Diagn
JT  - Prenatal diagnosis
JID - 8106540
SB  - IM
MH  - Adult
MH  - *Erythroblasts
MH  - Female
MH  - Fetal Blood/*cytology
MH  - *Gestational Age
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Middle Aged
MH  - Pregnancy
MH  - Pregnancy Trimester, First/*blood
MH  - Pregnancy Trimester, Second/*blood
MH  - Prenatal Diagnosis/*methods
EDAT- 2001/03/22 10:00
MHDA- 2001/07/20 10:01
CRDT- 2001/03/22 10:00
PHST- 2001/03/22 10:00 [pubmed]
PHST- 2001/07/20 10:01 [medline]
PHST- 2001/03/22 10:00 [entrez]
AID - 10.1002/1097-0223(200103)21:3<165::AID-PD29>3.0.CO;2-F [pii]
AID - 10.1002/1097-0223(200103)21:3<165::aid-pd29>3.0.co;2-f [doi]
PST - ppublish
SO  - Prenat Diagn. 2001 Mar;21(3):165-70. doi: 
      10.1002/1097-0223(200103)21:3<165::aid-pd29>3.0.co;2-f.