PMID- 11262393
OWN - NLM
STAT- MEDLINE
DCOM- 20010712
LR  - 20210209
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 276
IP  - 23
DP  - 2001 Jun 8
TI  - Calcium-dependent activation of nuclear factor regulated by interleukin 
      3/adenovirus E4 promoter-binding protein gene expression by calcineurin/nuclear 
      factor of activated T cells and calcium/calmodulin-dependent protein kinase 
      signaling.
PG  - 19921-8
AB  - An increase in the intracellular Ca(2+) concentration controls a diverse range of 
      cell functions, including gene expression, apoptosis, adhesion, motility, and 
      proliferation. We have investigated Ca(2+) regulation of gene expression in rat 
      aortic smooth muscle cells. We found that the expression of nuclear factor 
      regulated by interleukin 3 (NFIL3)/adenovirus E4 promoter-binding protein 
      (E4BP4)/basic region/leucine zipper (bZIP) type of a transcription factor that 
      has a very important function in cell survival, was activated by thapsigargin 
      (TG). This activation was inhibited by chelation of extra- or intracellular 
      Ca(2+), suggesting that the induction by TG was dependent on the elevation of 
      [Ca(2+)](i). Specific inhibition of calcineurin or calcium/calmodulin-dependent 
      protein kinase (CaM kinase) by chemical means impaired the TG-induced NFIL3/E4BP4 
      expression. Expression of dominant negative forms of calcineurin or nuclear 
      factor of activated T cells (NFAT) inhibited the induction of NFIL3/E4BP4 mRNA by 
      TG. These results suggest that intracellular Ca(2+) plays a critical role in 
      regulating gene expression of NFIL3/E4BP4 by calcineurin/NFAT and CaM kinase 
      signaling in vascular smooth muscle cells.
FAU - Nishimura, Y
AU  - Nishimura Y
AD  - Department of Molecular and Cellular Pharmacology, Mie University School of 
      Medicine, Edobashi, Tsu, Mie 514-8507, Japan.
FAU - Tanaka, T
AU  - Tanaka T
LA  - eng
SI  - GENBANK/AY004663
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20010321
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Basic-Leucine Zipper Transcription Factors)
RN  - 0 (DNA, Complementary)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (G-Box Binding Factors)
RN  - 0 (NFATC Transcription Factors)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Transcription Factors)
RN  - EC 2.7.11.17 (Calcium-Calmodulin-Dependent Protein Kinases)
RN  - EC 3.1.3.16 (Calcineurin)
RN  - EC 3.1.3.16 (Phosphoprotein Phosphatases)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Basic-Leucine Zipper Transcription Factors
MH  - Calcineurin/*physiology
MH  - Calcium/*physiology
MH  - Calcium-Calmodulin-Dependent Protein Kinases/antagonists & inhibitors/*metabolism
MH  - Cells, Cultured
MH  - DNA, Complementary
MH  - DNA-Binding Proteins/*genetics/physiology
MH  - Enzyme Inhibitors/pharmacology
MH  - G-Box Binding Factors
MH  - Gene Expression Regulation/*physiology
MH  - Lymphocyte Activation
MH  - Molecular Sequence Data
MH  - Muscle, Smooth, Vascular/cytology/enzymology/metabolism
MH  - NFATC Transcription Factors
MH  - *Nuclear Proteins
MH  - Phosphoprotein Phosphatases/antagonists & inhibitors
MH  - Rats
MH  - Sequence Homology, Amino Acid
MH  - Signal Transduction
MH  - T-Lymphocytes/enzymology/*metabolism
MH  - Transcription Factors/*genetics/physiology
MH  - Transcription, Genetic/physiology
EDAT- 2001/03/23 10:00
MHDA- 2001/07/13 10:01
CRDT- 2001/03/23 10:00
PHST- 2001/03/23 10:00 [pubmed]
PHST- 2001/07/13 10:01 [medline]
PHST- 2001/03/23 10:00 [entrez]
AID - S0021-9258(19)40423-7 [pii]
AID - 10.1074/jbc.M010332200 [doi]
PST - ppublish
SO  - J Biol Chem. 2001 Jun 8;276(23):19921-8. doi: 10.1074/jbc.M010332200. Epub 2001 
      Mar 21.