PMID- 11263185
OWN - NLM
STAT- MEDLINE
DCOM- 20010503
LR  - 20061115
IS  - 0529-567X (Print)
IS  - 0529-567X (Linking)
VI  - 34
IP  - 3
DP  - 1999 Mar
TI  - [Application of fluorescence insitu hybridization technique for prenatal 
      diagnosis of chromosome abnormality in amniotic cells].
PG  - 153-5
AB  - OBJECTIVE: To study the technique and diagnostic value of fluorescence in situ 
      hybridization (FISH) in chromosome abnormality for prenatal diagnosis. METHODS: 
      Amniocenteses were performed in 34 pregnant women of 16-23 gestational weeks with 
      indications for prenatal diagnosis. The amniotic fluid samples were cultured in 
      Chang's medium. The metaphase chromosomes were hybridized in situ with the human 
      centromere probes, alpha-satellites DNA probes of X, Y, 13, 21, 18 chromosomes 
      and all primer chromosome probes. These probes were conjugated by Biotin and 
      Dioxin then. The treated slides were examined and taken photos under the 
      fluoromicroscope. RESULTS: By FISH technique, normal karyotypes were shown in 31 
      cases: 16 cases of 46, XY, 15 cases of 46, XX. Abnormalities were found in 3 
      patients which were 47, XY + 21; 47, XY + 18 and 45, X/46, X, r (X) respectively. 
      CONCLUSION: Using FISH technique to detect chromosome abnormalities in amniotic 
      fluid for prenatal diagnosis is a reliable method which is rapid and accurate. It 
      has higher sensitivity and specificity in finding chromosome structure 
      abnormalities and marking chromosome DNA sequences.
FAU - Huang, Y
AU  - Huang Y
AD  - Second Municipal People's Hospital of Guangzhou, Guangzhou 510150.
FAU - Sun, X
AU  - Sun X
FAU - Li, Q
AU  - Li Q
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Zhonghua Fu Chan Ke Za Zhi
JT  - Zhonghua fu chan ke za zhi
JID - 16210370R
SB  - IM
MH  - Adult
MH  - Amniocentesis
MH  - Amniotic Fluid
MH  - Chromosome Aberrations/*genetics
MH  - Female
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Pregnancy
MH  - *Prenatal Diagnosis
MH  - Sex Chromosome Aberrations/diagnosis
MH  - X Chromosome
EDAT- 2001/03/27 10:00
MHDA- 2001/05/05 10:01
CRDT- 2001/03/27 10:00
PHST- 2001/03/27 10:00 [pubmed]
PHST- 2001/05/05 10:01 [medline]
PHST- 2001/03/27 10:00 [entrez]
PST - ppublish
SO  - Zhonghua Fu Chan Ke Za Zhi. 1999 Mar;34(3):153-5.