PMID- 11264239
OWN - NLM
STAT- MEDLINE
DCOM- 20010517
LR  - 20191210
IS  - 0007-1188 (Print)
IS  - 0007-1188 (Linking)
VI  - 132
IP  - 7
DP  - 2001 Apr
TI  - Heterogeneous increases of cytoplasmic calcium: distinct effects on 
      down-regulation of cell surface sodium channels and sodium channel subunit mRNA 
      levels.
PG  - 1455-66
AB  - 1. Long-term (> or = 12 h) treatment of cultured bovine adrenal chromaffin cells 
      with A23187 (a Ca(2+) ionophore) or thapsigargin (TG) [an inhibitor of 
      sarco(endo)plasmic reticulum Ca(2+)-ATPase (SERCA)] caused a time- and 
      concentration-dependent reduction of cell surface [(3)H]-saxitoxin (STX) binding 
      capacity, but did not change the K:(D:) value. In A23187- or TG-treated cells, 
      veratridine-induced (22)Na(+) influx was reduced (with no change in veratridine 
      EC(50) value) while it was enhanced by alpha-scorpion venom, beta-scorpion venom, 
      or Ptychodiscus brevis toxin-3, like in nontreated cells. 2. The A23187- or 
      TG-induced decrease of [(3)H]-STX binding was diminished by BAPTA-AM. EGTA also 
      inhibited the decreasing effect of A23187. A23187 caused a rapid, monophasic and 
      persistent increase in intracellular concentration of Ca(2+) ([Ca(2+)](i)) to a 
      greater extent than that observed with TG. 2,5-Di-(t-butyl)-1,4-benzohydroquinone 
      (DBHQ) (an inhibitor of SERCA) produced only a rapid monophasic increase in 
      [Ca(2+)](i), without any effect on [(3)H]-STX binding. 3. Reduction in [(3)H]-STX 
      binding capacity induced by A23187 or TG was attenuated by Go6976 (an inhibitor 
      of conventional protein kinase C) or calpastatin peptide (an inhibitor of 
      calpain). When the internalization rate of cell surface Na(+) channels was 
      measured in the presence of brefeldin A (an inhibitor of vesicular exit from the 
      trans-Golgi network), A23187 or TG accelerated the reduction of [(3)H]-STX 
      binding capacity. 4. Six hours treatment with A23187 lowered Na(+) channel alpha- 
      and beta(1)-subunit mRNA levels, whereas TG had no effect. 5. These results 
      suggest that elevation of [Ca(2+)](i) caused by A23187, TG or DBHQ exerted 
      differential effects on down-regulation of cell surface functional Na(+) channels 
      and Na(+) channel subunit mRNA levels.
FAU - Shiraishi, S
AU  - Shiraishi S
AD  - Department of Pharmacology, Miyazaki Medical College, Miyazaki 889-1692, Japan.
FAU - Shibuya, I
AU  - Shibuya I
FAU - Uezono, Y
AU  - Uezono Y
FAU - Yokoo, H
AU  - Yokoo H
FAU - Toyohira, Y
AU  - Toyohira Y
FAU - Yamamoto, R
AU  - Yamamoto R
FAU - Yanagita, T
AU  - Yanagita T
FAU - Kobayashi, H
AU  - Kobayashi H
FAU - Wada, A
AU  - Wada A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Br J Pharmacol
JT  - British journal of pharmacology
JID - 7502536
RN  - 0 (Calcium-Binding Proteins)
RN  - 0 (Carbazoles)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Hydroquinones)
RN  - 0 (Indoles)
RN  - 0 (Ionophores)
RN  - 0 (Marine Toxins)
RN  - 0 (Oxocins)
RN  - 0 (Protein Subunits)
RN  - 0 (RNA, Messenger)
RN  - 0 (Scorpion Venoms)
RN  - 0 (Sodium Channels)
RN  - 10028-17-8 (Tritium)
RN  - 136194-77-9 (Go 6976)
RN  - 139890-68-9 (1,2-bis(2-aminophenoxy)ethane N,N,N',N'-tetraacetic acid 
      acetoxymethyl ester)
RN  - 20350-15-6 (Brefeldin A)
RN  - 26XK13B61B (2,5-di-tert-butylhydroquinone)
RN  - 35523-89-8 (Saxitoxin)
RN  - 37H9VM9WZL (Calcimycin)
RN  - 526U7A2651 (Egtazic Acid)
RN  - 67526-95-8 (Thapsigargin)
RN  - 71-62-5 (Veratridine)
RN  - 79079-11-1 (calpastatin)
RN  - 85079-48-7 (brevetoxin T17)
RN  - 9NEZ333N27 (Sodium)
RN  - EC 3.4.22.- (Calpain)
RN  - EC 3.6.3.8 (Sarcoplasmic Reticulum Calcium-Transporting ATPases)
RN  - EC 7.2.2.10 (Calcium-Transporting ATPases)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Animals
MH  - Binding, Competitive/drug effects
MH  - Brefeldin A/pharmacology
MH  - Calcimycin/pharmacology
MH  - Calcium/*metabolism/pharmacology
MH  - Calcium-Binding Proteins/pharmacology
MH  - Calcium-Transporting ATPases/antagonists & inhibitors
MH  - Calpain/antagonists & inhibitors
MH  - Carbazoles/pharmacology
MH  - Cattle
MH  - Cells, Cultured
MH  - Chromaffin Cells/drug effects/metabolism
MH  - Dose-Response Relationship, Drug
MH  - Down-Regulation
MH  - Egtazic Acid/analogs & derivatives/pharmacology
MH  - Enzyme Inhibitors/pharmacology
MH  - Hydroquinones/pharmacology
MH  - Indoles/pharmacology
MH  - Ionophores/pharmacology
MH  - Marine Toxins/pharmacology
MH  - *Oxocins
MH  - Protein Subunits
MH  - RNA, Messenger/drug effects/genetics/metabolism
MH  - Sarcoplasmic Reticulum Calcium-Transporting ATPases
MH  - Saxitoxin/metabolism
MH  - Scorpion Venoms/pharmacology
MH  - Sodium/metabolism
MH  - Sodium Channels/drug effects/genetics/*metabolism
MH  - Thapsigargin/pharmacology
MH  - Time Factors
MH  - Tritium
MH  - Veratridine/pharmacology
PMC - PMC1572695
EDAT- 2001/03/27 10:00
MHDA- 2001/05/18 10:01
PMCR- 2002/04/01
CRDT- 2001/03/27 10:00
PHST- 2001/03/27 10:00 [pubmed]
PHST- 2001/05/18 10:01 [medline]
PHST- 2001/03/27 10:00 [entrez]
PHST- 2002/04/01 00:00 [pmc-release]
AID - 0703960 [pii]
AID - 10.1038/sj.bjp.0703960 [doi]
PST - ppublish
SO  - Br J Pharmacol. 2001 Apr;132(7):1455-66. doi: 10.1038/sj.bjp.0703960.