PMID- 11264540
OWN - NLM
STAT- MEDLINE
DCOM- 20010412
LR  - 20070319
IS  - 0036-8075 (Print)
IS  - 0036-8075 (Linking)
VI  - 291
IP  - 5512
DP  - 2001 Mar 23
TI  - Activity-dependent transfer of brain-derived neurotrophic factor to postsynaptic 
      neurons.
PG  - 2419-23
AB  - Neurotrophins such as brain-derived neurotrophic factor (BDNF) are thought to be 
      transferred from post- to presynaptic neurons and to be involved in the formation 
      and plasticity of neural circuits. However, direct evidence for a transneuronal 
      transfer of BDNF and its relation to neuronal activity remains elusive. We 
      simultaneously injected complementary DNAs of green fluorescent protein 
      (GFP)-tagged BDNF and red fluorescence protein into the nucleus of single neurons 
      and visualized expression, localization, and transport of BDNF in living neurons. 
      Fluorescent puncta representing BDNF moved in axons in the anterograde direction, 
      though some moved retrogradely, and transferred to postsynaptic neurons in an 
      activity-dependent manner.
FAU - Kohara, K
AU  - Kohara K
AD  - Division of Neurophysiology, Biomedical Research Center, Osaka University 
      Graduate School of Medicine, 2-2 Yamadaoka, Suita, Osaka 565-0871 Japan.
FAU - Kitamura, A
AU  - Kitamura A
FAU - Morishima, M
AU  - Morishima M
FAU - Tsumoto, T
AU  - Tsumoto T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Science
JT  - Science (New York, N.Y.)
JID - 0404511
RN  - 0 (Antibodies)
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (DNA, Complementary)
RN  - 0 (Luminescent Proteins)
RN  - 0 (Microtubule-Associated Proteins)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (fluorescent protein 583)
RN  - 0 (tau Proteins)
RN  - 4368-28-9 (Tetrodotoxin)
SB  - IM
MH  - Animals
MH  - Animals, Newborn
MH  - Antibodies
MH  - *Axonal Transport
MH  - Axons/*metabolism
MH  - Brain-Derived Neurotrophic Factor/genetics/*metabolism
MH  - Cell Nucleus/metabolism
MH  - Cells, Cultured
MH  - DNA, Complementary
MH  - Dendrites/metabolism
MH  - Immunohistochemistry
MH  - Luminescent Proteins/metabolism
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Microscopy, Fluorescence
MH  - Microtubule-Associated Proteins/analysis/immunology
MH  - Neurites/metabolism
MH  - Neuronal Plasticity
MH  - Neurons/drug effects/*metabolism
MH  - Plasmids
MH  - Protein Transport
MH  - Recombinant Fusion Proteins/metabolism
MH  - Synapses/*metabolism
MH  - Tetrodotoxin/pharmacology
MH  - Visual Cortex/cytology
MH  - tau Proteins/analysis/immunology
EDAT- 2001/03/27 10:00
MHDA- 2001/04/17 10:01
CRDT- 2001/03/27 10:00
PHST- 2001/03/27 10:00 [pubmed]
PHST- 2001/04/17 10:01 [medline]
PHST- 2001/03/27 10:00 [entrez]
AID - 291/5512/2419 [pii]
AID - 10.1126/science.1057415 [doi]
PST - ppublish
SO  - Science. 2001 Mar 23;291(5512):2419-23. doi: 10.1126/science.1057415.