PMID- 11266086
OWN - NLM
STAT- MEDLINE
DCOM- 20010726
LR  - 20061115
IS  - 0004-069X (Print)
IS  - 0004-069X (Linking)
VI  - 49
IP  - 1
DP  - 2001
TI  - Phage display as a tool for rapid cloning of allergenic proteins.
PG  - 19-25
AB  - Allergic diseases represent an immune disorder associated with the production of 
      immunoglobulin E (IgE) against normally innocuous antigens (allergens). Almost 
      20% of the population in industrialized countries suffer from type I allergic 
      symptoms such as allergic rhinitis, conjunctivitis, urticaria or asthma. Although 
      the mechanisms responsible for these allergic reactions are quite well 
      understood, knowledge about the repertoire of molecules able to elicit type I 
      symptoms is still limited. To clone and characterize entire allergen repertoires 
      from complex allergenic sources in a fast and efficient way, new technologies are 
      required. The phage surface display of cDNA libraries described here has proven 
      to be a versatile cloning system to selectively isolate allergens physically 
      linked to their genetic information. The screening of cDNA libraries displayed on 
      phage surfaces with immobilised serum IgE from allergic patients reduces the time 
      required for the selection of candidate clones to a few weeks. Robot-assisted 
      high-throughput screening of the enriched library provides a fast and 
      cost-effective way to isolate complete allergen repertoires. The biotechnological 
      production of recombinant allergens derived from these sequences bears a high 
      potential for the improvement of the diagnosis of allergic diseases.
FAU - Appenzeller, U
AU  - Appenzeller U
AD  - Swiss Institute of Allergy and Asthma Research, Davos.
FAU - Blaser, K
AU  - Blaser K
FAU - Crameri, R
AU  - Crameri R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Review
PL  - Poland
TA  - Arch Immunol Ther Exp (Warsz)
JT  - Archivum immunologiae et therapiae experimentalis
JID - 0114365
RN  - 0 (Allergens)
RN  - 0 (DNA, Complementary)
RN  - 0 (Peptide Library)
RN  - 0 (Recombinant Proteins)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Allergens/*genetics
MH  - Cloning, Molecular
MH  - DNA, Complementary/genetics
MH  - Genetic Vectors
MH  - Humans
MH  - Hypersensitivity, Immediate/immunology
MH  - Immunoglobulin E/blood
MH  - *Peptide Library
MH  - Recombinant Proteins/genetics
RF  - 36
EDAT- 2001/03/27 10:00
MHDA- 2001/07/28 10:01
CRDT- 2001/03/27 10:00
PHST- 2001/03/27 10:00 [pubmed]
PHST- 2001/07/28 10:01 [medline]
PHST- 2001/03/27 10:00 [entrez]
PST - ppublish
SO  - Arch Immunol Ther Exp (Warsz). 2001;49(1):19-25.