PMID- 11266284
OWN - NLM
STAT- MEDLINE
DCOM- 20010503
LR  - 20161124
IS  - 0022-4804 (Print)
IS  - 0022-4804 (Linking)
VI  - 96
IP  - 2
DP  - 2001 Apr
TI  - Autologous bone marrow implantation induced angiogenesis and improved 
      deteriorated exercise capacity in a rat ischemic hindlimb model.
PG  - 277-83
AB  - BACKGROUND: Bone marrow possesses endothelial progenitor cells that secrete 
      several growth factors and can contribute to the formation of new capillaries. In 
      the present study, we investigated the extent of angiogenesis induced by 
      implantation of autologous bone marrow cells (BMCs) in a rat ischemic hindlimb 
      model and studied whether the increased collateral vessels can improve 
      deteriorated physical function. MATERIALS AND METHODS: Ischemic hindlimb was 
      created by ligation of the femoral artery and its branches in Dark Agouti (DA) 
      rats. BMCs (1 x 10(7)) were injected percutaneously at six points into the 
      gastrocnemius muscle. To assess angiogenesis, histologic evaluation and 
      microangiography were performed at 2 weeks postligation. Severity of the ischemic 
      insult was evaluated by measuring blood flow in the adductor and gastrocnemius 
      muscles using nonradioactive colored microspheres and by determining the femoral 
      arteriovenous oxygen difference (AVDO(2)) at 2 weeks postligation. Running time 
      on a motor-driven treadmill was used to represent exercise capacity. RESULTS: The 
      histologic evaluation and microangiogram showed that the implanted BMCs induce 
      angiogenesis. Blood flow to the adductor muscle on the treated side in the bone 
      marrow cell implantation (BMI) group was significantly restored to 77.3 +/- 19.3% 
      of that of the normally perfused limb in comparison to that in control groups (P 
      < 0.05). AVDO(2) in the BMI group significantly decreased when compared with 
      AVDO(2) in control groups. Rats in the BMI group ran approximately 1.5 times 
      longer than rats in control groups at 2 and 4 weeks postligation (P < 0.01). 
      CONCLUSIONS: Implantation of autologous BMCs induced angiogenesis and improved 
      deteriorated exercise capacity in our rat ischemic hindlimb model.
CI  - Copyright 2001 Academic Press.
FAU - Ikenaga, S
AU  - Ikenaga S
AD  - First Department of Surgery, First Department of Physiology, Second Department of 
      Internal Medicine, Yamaguchi University School of Medicine, 1-1-1 Minami-Kogushi, 
      Ube, Yamaguchi, 755-8505, Japan.
FAU - Hamano, K
AU  - Hamano K
FAU - Nishida, M
AU  - Nishida M
FAU - Kobayashi, T
AU  - Kobayashi T
FAU - Li, T S
AU  - Li TS
FAU - Kobayashi, S
AU  - Kobayashi S
FAU - Matsuzaki, M
AU  - Matsuzaki M
FAU - Zempo, N
AU  - Zempo N
FAU - Esato, K
AU  - Esato K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Surg Res
JT  - The Journal of surgical research
JID - 0376340
SB  - IM
MH  - Angiography
MH  - Animals
MH  - *Bone Marrow Transplantation
MH  - Forelimb/blood supply
MH  - Hindlimb/*blood supply/diagnostic imaging
MH  - Ischemia/*physiopathology/*surgery
MH  - Male
MH  - Motor Activity/physiology
MH  - Muscle, Skeletal/blood supply/pathology
MH  - Neovascularization, Physiologic/*physiology
MH  - Oxygen Consumption
MH  - *Physical Endurance
MH  - Rats
MH  - Regional Blood Flow
MH  - Time Factors
MH  - Transplantation, Autologous
EDAT- 2001/03/27 10:00
MHDA- 2001/05/05 10:01
CRDT- 2001/03/27 10:00
PHST- 2001/03/27 10:00 [pubmed]
PHST- 2001/05/05 10:01 [medline]
PHST- 2001/03/27 10:00 [entrez]
AID - S0022-4804(00)96080-3 [pii]
AID - 10.1006/jsre.2000.6080 [doi]
PST - ppublish
SO  - J Surg Res. 2001 Apr;96(2):277-83. doi: 10.1006/jsre.2000.6080.