PMID- 11269345
OWN - NLM
STAT- MEDLINE
DCOM- 20010517
LR  - 20190915
IS  - 0831-2796 (Print)
IS  - 0831-2796 (Linking)
VI  - 44
IP  - 1
DP  - 2001 Feb
TI  - Sau3A in situ digestion of human chromosome 3 pericentrometric heterochromatin. 
      I. Differential digestion of alpha-satellite and satellite 1 DNA sequences.
PG  - 120-7
AB  - In situ digestion with the restriction endonuclease (RE) Sau3A (Sau3A REISD) 
      uncovers a polymorphism for the pericentromeric heterochromatin of human 
      chromosome 3, which can be positively stained (3+) or not (3-), and has proven 
      useful to differentiate donor and recipient cells after sex-matched bone marrow 
      transplantation and to analyze the so-called hemopoietic chimerism. The aim of 
      the present investigation was to obtain insight into the molecular basis of such 
      polymorphism to optimize its use for chimerism quantification using 
      methodological approaches other than REISD. To this end, fluorescence in situ 
      hybridization (FISH) assays using probes for the satellite DNA sequences that 
      mainly constitute chromosome 3 pericentromeric heterochromatin (alpha-satellite 
      and satellite 1 DNA) were performed on control and Sau3A-digested chromosomes. 
      The results obtained suggest that chromosome 3 alpha-satellite DNA is digested in 
      all individuals studied, irrespective of the karyotype obtained by Sau3A REISD 
      (3++, 3+-, 3--), and thus it does not seem to be involved in the polymorphism 
      uncovered by Sau3A on this chromosome. Satellite 1 DNA is not digested in any 
      case, and shows a polymorphism for its domain size, which correlates with the 
      polymorphism uncovered by Sau3A in such a way that 3+ chromosomes show a large 
      domain (3L) and 3- chromosomes show a small domain (3S). It seems, therefore, 
      that the cause of the polymorphism uncovered by Sau3A on the pericentromeric 
      region of chromosome 3 is a difference in the size of the satellite 1 DNA domain. 
      Small satellite 1 DNA domains fall under the resolution level of REISD technique 
      and are identified as 3-.
FAU - Buno, I
AU  - Buno I
AD  - Unidad de Trasplante de Medula Osea, Hospital GU Gregorio Maranon, Madrid, Spain. 
      ibuno@hotmail.com
FAU - Fernandez, J L
AU  - Fernandez JL
FAU - Lopez-Fernandez, C
AU  - Lopez-Fernandez C
FAU - Diez-Martin, J L
AU  - Diez-Martin JL
FAU - Gosalvez, J
AU  - Gosalvez J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Canada
TA  - Genome
JT  - Genome
JID - 8704544
RN  - 0 (DNA, Satellite)
RN  - 0 (Heterochromatin)
RN  - EC 3.1.21.4 (Deoxyribonucleases, Type II Site-Specific)
RN  - EC 3.1.21.4 (GATC-specific type II deoxyribonucleases)
SB  - IM
MH  - *Centromere
MH  - *Chromosomes, Human, Pair 3
MH  - DNA, Satellite/*genetics/metabolism
MH  - Deoxyribonucleases, Type II Site-Specific/*metabolism
MH  - Female
MH  - Heterochromatin/*metabolism
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Polymorphism, Genetic
EDAT- 2001/03/28 10:00
MHDA- 2001/05/18 10:01
CRDT- 2001/03/28 10:00
PHST- 2001/03/28 10:00 [pubmed]
PHST- 2001/05/18 10:01 [medline]
PHST- 2001/03/28 10:00 [entrez]
AID - 10.1139/gen-44-1-120 [doi]
PST - ppublish
SO  - Genome. 2001 Feb;44(1):120-7. doi: 10.1139/gen-44-1-120.