PMID- 11271781
OWN - NLM
STAT- MEDLINE
DCOM- 20011025
LR  - 20230307
IS  - 0362-028X (Print)
IS  - 0362-028X (Linking)
VI  - 64
IP  - 2
DP  - 2001 Feb
TI  - Effects of gamma radiation on the allergenic and antigenic properties of milk 
      proteins.
PG  - 272-6
AB  - This study was carried out to evaluate the application of food irradiation 
      technology as a method for reducing milk allergies. Bovine alpha-casein (ACA) and 
      beta-lactoglobulin (BLG) were used as milk proteins. Using milk-hypersensitive 
      patients' immunoglobulin E (IgE) and rabbit IgGs individually produced to ACA and 
      BLG, the changes of allergenicity and antigenicity of irradiated proteins were 
      observed by competitive indirect enzyme-linked immunosorbent assay. Allergenicity 
      and antigenicity of the irradiated proteins were changed with different slopes of 
      the inhibition curves. The disappearance of the band on sodium dodecyl 
      sulfate-polyacrylamide gel electrophoresis and increase of the turbidity showed 
      that solubility of the proteins decreased by radiation, and this decrease might 
      be caused by agglomeration of the proteins. These results indicated that epitopes 
      on milk allergens were structurally altered by gamma irradiation.
FAU - Lee, J W
AU  - Lee JW
AD  - The Team for Radiation Food Science and Biotechnology, Korea Atomic Energy 
      Research Institute, Yusong, Taejon.
FAU - Kim, J H
AU  - Kim JH
FAU - Yook, H S
AU  - Yook HS
FAU - Kang, K O
AU  - Kang KO
FAU - Lee, S Y
AU  - Lee SY
FAU - Hwang, H J
AU  - Hwang HJ
FAU - Byun, M W
AU  - Byun MW
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Food Prot
JT  - Journal of food protection
JID - 7703944
RN  - 0 (Allergens)
RN  - 0 (Caseins)
RN  - 0 (Immunoglobulin G)
RN  - 0 (Lactoglobulins)
RN  - 0 (Milk Proteins)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Allergens/chemistry/immunology/*radiation effects
MH  - Animals
MH  - Caseins/immunology/radiation effects
MH  - Cattle
MH  - Dose-Response Relationship, Radiation
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Enzyme-Linked Immunosorbent Assay
MH  - *Food Irradiation
MH  - Gamma Rays
MH  - Humans
MH  - Immunoglobulin E/immunology/metabolism/radiation effects
MH  - Immunoglobulin G/metabolism/radiation effects
MH  - Lactoglobulins/immunology/radiation effects
MH  - Milk Hypersensitivity/*prevention & control
MH  - Milk Proteins/immunology/metabolism/*radiation effects
MH  - Protein Binding/radiation effects
MH  - Rabbits
EDAT- 2001/03/29 10:00
MHDA- 2001/10/26 10:01
CRDT- 2001/03/29 10:00
PHST- 2001/03/29 10:00 [pubmed]
PHST- 2001/10/26 10:01 [medline]
PHST- 2001/03/29 10:00 [entrez]
AID - S0362-028X(22)00804-3 [pii]
AID - 10.4315/0362-028x-64.2.272 [doi]
PST - ppublish
SO  - J Food Prot. 2001 Feb;64(2):272-6. doi: 10.4315/0362-028x-64.2.272.