PMID- 11272275
OWN - NLM
STAT- MEDLINE
DCOM- 20010329
LR  - 20131121
IS  - 0741-5400 (Print)
IS  - 0741-5400 (Linking)
VI  - 69
IP  - 2
DP  - 2001 Feb
TI  - Increase of CCR1 and CCR5 expression and enhanced functional response to MIP-1 
      alpha during differentiation of human monocytes to macrophages.
PG  - 248-52
AB  - Chemokines and their receptors regulate migration of leukocytes under normal and 
      inflammatory conditions. In this study, we analyzed the CC chemokine receptor 
      (CCR) expression of monocytes differentiating in vitro to macrophages. We 
      observed a time-dependent change of expression and functional responsiveness of 
      CCR1, CCR2, and CCR5 within 48 h. Whereas freshly harvested monocytes were 
      strongly attracted by monocyte chemotactic protein 1 (MCP-1), a specific ligand 
      for CCR2, only a weak response was observed to macrophage inflammatory protein 
      1alpha (MIP-1alpha), which binds to CCR1 and CCR5. In striking contrast, 
      differentiated macrophages displayed a strong chemotactic response to MIP-1alpha 
      and only a weak response to MCP-1. These findings were paralleled by 
      intracellular calcium shifts. During the time course of monocyte to macrophage 
      differentiation, mRNA levels and surface expression of CCR2 decreased, whereas 
      that of CCR1 and CCR5 increased. The time-dependent switch from CCR2 on monocytes 
      to CCR1 and CCR5 on mature macrophages reflects a functional change belonging to 
      the differentiation process of monocytes to macrophages and may form the basis 
      for a differential responsiveness of monocytes and macrophages to distinct sets 
      of chemokines.
FAU - Kaufmann, A
AU  - Kaufmann A
AD  - Institute of Immunology, Philipps University, Marburg, Germany. 
      kaufmana@mailer.uni-marburg.de
FAU - Salentin, R
AU  - Salentin R
FAU - Gemsa, D
AU  - Gemsa D
FAU - Sprenger, H
AU  - Sprenger H
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Leukoc Biol
JT  - Journal of leukocyte biology
JID - 8405628
RN  - 0 (CCR1 protein, human)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemokine CCL3)
RN  - 0 (Chemokine CCL4)
RN  - 0 (Macrophage Inflammatory Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, CCR1)
RN  - 0 (Receptors, CCR5)
RN  - 0 (Receptors, Chemokine)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Calcium/metabolism
MH  - Cell Differentiation/immunology
MH  - Cells, Cultured
MH  - Chemokine CCL2/pharmacology
MH  - Chemokine CCL3
MH  - Chemokine CCL4
MH  - Humans
MH  - Macrophage Inflammatory Proteins/*pharmacology
MH  - Macrophages/*cytology/immunology/metabolism
MH  - Monocytes/*cytology/immunology/metabolism
MH  - RNA, Messenger/biosynthesis
MH  - Receptors, CCR1
MH  - Receptors, CCR5/*biosynthesis/genetics/physiology
MH  - Receptors, Chemokine/*biosynthesis/genetics/physiology
MH  - Time Factors
EDAT- 2001/03/29 10:00
MHDA- 2001/04/03 10:01
CRDT- 2001/03/29 10:00
PHST- 2001/03/29 10:00 [pubmed]
PHST- 2001/04/03 10:01 [medline]
PHST- 2001/03/29 10:00 [entrez]
PST - ppublish
SO  - J Leukoc Biol. 2001 Feb;69(2):248-52.