PMID- 11272892
OWN - NLM
STAT- MEDLINE
DCOM- 20010329
LR  - 20191210
IS  - 1525-1578 (Print)
IS  - 1525-1578 (Linking)
VI  - 2
IP  - 2
DP  - 2000 May
TI  - Concomitant oncoprotein detection with fluorescence in situ hybridization 
      (CODFISH): a fluorescence-based assay enabling simultaneous visualization of gene 
      amplification and encoded protein expression.
PG  - 78-83
AB  - We sought the validation of a three-color fluorescence-based system that 
      simultaneously profiles Her2/neu oncogene copy by fluorescence in situ 
      hybridization (FISH) and Her-2/neu encoded protein by the use of a versatile 
      alkaline phosphatase chromogen fast red K in either fluorescence or bright-field 
      mode. Nuclei were counterstained with DAPI. Nineteen infiltrating ductal 
      carcinomas of breast were comprehensively evaluated for Her-2/neu 
      amplification/overexpression by direct and indirect FISH using digoxigenin 
      (DigFISH) and direct fluorescently labeled probes, autoradiographic RNA:RNA in 
      situ hybridization, and immunohistochemistry using monoclonal antibody CB11. 
      CODFISH results correlated well with DigFISH, direct-label FISH, mRNA expression, 
      and oncoprotein expression as assessed with CB11, and enabled simultaneous 
      visualization of gene copy and protein. In addition, qualitative 
      immunohistochemistry may be followed by CODFISH gene copy enumeration to clarify 
      ambiguous cases.
FAU - Tubbs, R R
AU  - Tubbs RR
AD  - Department of Clinical Pathology, the Cleveland Clinic Foundation, Ohio 44195, 
      USA. TubbsR@ccf.org
FAU - Pettay, J
AU  - Pettay J
FAU - Roche, P
AU  - Roche P
FAU - Stoler, M H
AU  - Stoler MH
FAU - Jenkins, R
AU  - Jenkins R
FAU - Myles, J
AU  - Myles J
FAU - Grogan, T
AU  - Grogan T
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Validation Study
PL  - United States
TA  - J Mol Diagn
JT  - The Journal of molecular diagnostics : JMD
JID - 100893612
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (RNA, Neoplasm)
RN  - EC 2.7.10.1 (Receptor, ErbB-2)
SB  - IM
MH  - Antibodies, Monoclonal
MH  - Breast Neoplasms/genetics/metabolism
MH  - Carcinoma, Ductal, Breast/genetics/metabolism
MH  - Female
MH  - Gene Amplification
MH  - Gene Expression
MH  - *Genes, erbB-2
MH  - Humans
MH  - Immunohistochemistry
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - RNA, Neoplasm/genetics/metabolism
MH  - Receptor, ErbB-2/genetics/metabolism
PMC - PMC1906899
EDAT- 2001/03/29 10:00
MHDA- 2001/04/03 10:01
PMCR- 2001/05/01
CRDT- 2001/03/29 10:00
PHST- 2001/03/29 10:00 [pubmed]
PHST- 2001/04/03 10:01 [medline]
PHST- 2001/03/29 10:00 [entrez]
PHST- 2001/05/01 00:00 [pmc-release]
AID - S1525-1578(10)60620-4 [pii]
AID - 0019 [pii]
AID - 10.1016/S1525-1578(10)60620-4 [doi]
PST - ppublish
SO  - J Mol Diagn. 2000 May;2(2):78-83. doi: 10.1016/S1525-1578(10)60620-4.